产品名称
抗脑源性神经营养因子抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... BDNF(627)
Application
免疫组化： 5-10 μg/mL（请参阅建议的方案）。
免疫印迹： 5-10μg/mL。 BDNF在标准缓冲液中不易提取。切开的组织应在10倍体积的100mM磷酸盐缓冲液中匀浆，该缓冲液包含1mM EDTA，2M盐酸胍（pH7.2）和三种蛋白酶抑制剂，10mM N-乙基马来酰亚胺，0.36 mM胃抑素和1mM PMSF。然后对匀浆进行超声处理，并在4℃下以46K x g离心30分钟。 BDNF迁移为18 kDa，同型二聚体约为27-30kDa。1:1,000
ELISA： 5-10μg/mL
生物活性：中和BDNF，但不中和其他神经营养因子。
最佳工作稀释度必须由最终用户进行确定。
免疫印迹： 5-10μg/mL。 BDNF在标准缓冲液中不易提取。切开的组织应在10倍体积的100mM磷酸盐缓冲液中匀浆，该缓冲液包含1mM EDTA，2M盐酸胍（pH7.2）和三种蛋白酶抑制剂，10mM N-乙基马来酰亚胺，0.36 mM胃抑素和1mM PMSF。然后对匀浆进行超声处理，并在4℃下以46K x g离心30分钟。 BDNF迁移为18 kDa，同型二聚体约为27-30kDa。1:1,000
ELISA： 5-10μg/mL
生物活性：中和BDNF，但不中和其他神经营养因子。
最佳工作稀释度必须由最终用户进行确定。
抗脑源性神经营养因子抗体可检测脑源性神经营养因子水平 & 已出版 & 并经过验证可用于ELISA、WB、IH。
研究子类别
神经化学 & 神经营养因子
神经炎症 & 疼痛
神经化学 & 神经营养因子
神经炎症 & 疼痛
研究类别
神经科学
神经科学
Biochem/physiol Actions
脑源性神经营养因子（BDNF）。通过斑点印迹，与NGF、NT3或NT4的交叉反应性小于1%。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
重组人脑源性神经营养因子（BDNF）。
Other Notes
替代品：AB1534P
Physical form
从PBS冻干的免疫球蛋白级分，不含防腐剂。用500 μL无菌去离子水复溶（1 mg/mL）。
形式：纯化
Preparation Note
接收后，冻干材料可在-20至-70°C下保存长达1年。复溶后，以未稀释等分试样的形式在-20至-70°C下保存长达6个月。可添加（1:1）甘油（ACS级或更优级）以获得额外的稳定性。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
E M Nikulina et al.
Neuroscience, 212, 38-48 (2012-04-24)
Intermittent social defeat stress exposure augments behavioral response to psychostimulants in a process termed cross-sensitization. Brain-derived neurotrophic factor (BDNF) mediates synaptic plasticity and cellular responses to stress and drugs of abuse. We previously showed that repeated social defeat stress persistently
Nicolás Unsain et al.
Journal of neurochemistry, 111(2), 428-440 (2009-08-19)
Brain-derived neurotrophic factor (BDNF) is involved in many aspects of neuronal biology and hippocampal physiology. Status epilepticus (SE) is a condition in which prolonged seizures lead to neuronal degeneration. SE-induced in rodents serves as a model of Temporal Lobe Epilepsy
Junshi Wang et al.
Neuropharmacology, 109, 121-130 (2016-05-08)
Stress is a major risk factor for substance abuse. Intermittent social defeat stress increases drug self-administration (SA) and elevates brain-derived neurotrophic factor (BDNF) expression in the ventral tegmental area (VTA) in rats. Intra-VTA BDNF overexpression enhances social defeat stress-induced cross-sensitization
Alfonso Paredes et al.
Developmental biology, 267(2), 430-449 (2004-03-12)
Although it is well established that both follicular assembly and the initiation of follicle growth in the mammalian ovary occur independently of pituitary hormone support, the factors controlling these processes remain poorly understood. We now report that neurotrophins (NTs) signaling
BDNF overexpression in the ventral tegmental area prolongs social defeat stress-induced cross-sensitization to amphetamine and increases ?FosB expression in mesocorticolimbic regions of rats.
Wang, J; Fanous, S; Terwilliger, EF; Bass, CE; Hammer, RP; Nikulina, EM
Neuropsychopharmacology null
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