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PICM0RG50

Millicell器官培养型站立式培养小室, 亲水PTFE膜, 0.4 µm, 30 mm, 50/pk

Name: Millicell器官培养型站立式培养小室, 亲水PTFE膜, 0.4 µm, 30 mm, 50/pk
Brand: MM

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, size 6 wells, sterile

别名:

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, cell culture inserts, organotypic inserts, plate inserts

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About This Item

UNSPSC代码:

41104923

eCl@ss:

32011202

NACRES:

NB.22

物料

polystyrene housing
transparent PTFE membrane

质量水平

400

无菌性

ethylene oxide treated
sterile

特点

hydrophilic

制造商/商品名称

Millicell^®

包装

pkg of Individually blister packaged

参数

50 °C max. temp.

技术

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

高度

5 mm

直径

30 mm

尺寸

6 wells

表面积

4.2 cm²

颜色

transparent, when wetted

基质

Biopore^™

孔径

0.4 μm pore size

吸附类型

low binding surface

检测方法

fluorometric

运输

ambient

一般描述

凭借Millicell嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。另外，Millicell嵌套板可用于从两侧研究单层细胞。

应用

细胞附着、3D 细胞培养、细胞生长、细胞分化、免疫细胞化学
Biopore 膜（亲水PTFE）是低蛋白结合、活细胞观察和免疫荧光应用的理想选择。

特点和优势

三维外植体结构的高活力和优越性研究
较低的高度令其适合装入标准培养皿
Biopore (PTFE) 膜提供高活力—长达40天—和良好的跨膜氧转运
该膜是透明的，并针对器官型外植体的长期维护进行优化。

法律信息

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

CLS430166

Corning^® 经组织培养处理的培养皿, D × H 60 mm × 15 mm

PICM03050

Millicell^® Standing细胞培养池, pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 13 mm, size 6 wells, sterile

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Circadian Regulation of IOP Rhythm by Dual Pathways of Glucocorticoids and the Sympathetic Nervous System.

Keisuke Ikegami et al.

Investigative ophthalmology & visual science, 61(3), 26-26 (2020-03-18)

Elevated IOP can cause the development of glaucoma. The circadian rhythm of IOP depends on the dynamics of the aqueous humor and is synchronized with the circadian rhythm pacemaker, that is, the suprachiasmatic nucleus. The suprachiasmatic nucleus resets peripheral clocks

Gene Therapy Using Neural Stem/Progenitor Cells Derived from Human Induced Pluripotent Stem Cells: Visualization of Migration and Bystander Killing Effect.

Ryota Tamura et al.

Human gene therapy, 31(5-6), 352-366 (2020-02-23)

Glioblastoma is the most aggressive brain tumor characterized by diffuse infiltration into the normal brain parenchyma. Neural stem cells are known to possess the tumor-tropic migratory capacity and thus can be used as cellular vehicles for targeted delivery of therapeutic

JNK signaling is required for proper tangential migration and laminar allocation of cortical interneurons.

Abigail K Myers et al.

Development (Cambridge, England), 147(2) (2020-01-10)

The precise migration of cortical interneurons is essential for the formation and function of cortical circuits, and disruptions to this key developmental process are implicated in the etiology of complex neurodevelopmental disorders, including schizophrenia, autism and epilepsy. We have recently

Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.

Spooner, B S, et al.

The Journal of Experimental Zoology, 269, 212-222 (1994)

Growth and morphogenesis of embryonic mouse organs on non-coated and extracellular matrix-coated Biopore membrane.

P Hardman et al.

Development, growth & differentiation, 35(6), 683-690 (1993-01-01)

Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane

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实验方案

Toluidine Blue Staining Protocol for Millicell^® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Basic Indirect Co-culture on Both Sides of Millicell^® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

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