推荐产品
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
BDNF129-13, monoclonal
形式
buffered aqueous solution
分子量
~12 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunoblotting: 2-4 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein
immunocytochemistry: suitable
immunohistochemistry: 20-40 μg/mL using pronase retrieved formalin-fixed, paraffin-embedded human cerebellum sections
immunoprecipitation (IP): 10-20 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... BDNF(627)
一般描述
Anti-BDNF antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the BDNF129-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Brain-derived neurotrophic factor (BDNF), also known as Abrineurin, is a secreted dimeric protein, which belongs to the neurotrophin family of growth factors, along with nerve growth factor (NGF) and neurotrophins 3, 4/5 and 6. BDNF is widely expressed in the central nerve system (CNS).
免疫原
Synthetic peptide corresponding to a region located in the mature human BDNF protein conjugated to KLH
应用
Anti-BDNF antibody, Mouse Monoclonal has been used in:
- immunoblotting
- immunohistochemistry
- immunoprecipitation
- immunocytochemistry
生化/生理作用
Brain-derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. BDNF also plays a vital role in vascular function and participates in angiogenesis through the specific receptor tropomyosin-related kinase B (TrkB). Changes in BDNF expression have been extensively investigated in depression, drug addition, schizophrenia, bipolar and anxiety disorders, Huntington′s disease and Rett syndrome. In addition, BDNF is associated with Alzheimer′s disease (AD) and its reduced levels were detected in post-mortem brain tissues of AD patients. It can exert profound effects on development, morphology, synaptic plasticity and brain functions.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Brain-derived neurotrophic factor and its clinical implications
Bathina S and Das UN
Archives of Medical Science : Ams, 11(6), 1164-1164 (2015)
Patricia Sampedro-Piquero et al.
Addiction biology, 27(2), e13158-e13158 (2022-03-02)
Our aim was to assess the cognitive and emotional state, as well as related-changes in the glucocorticoid receptor (GR), the corticotropin-releasing factor (CRF) and the brain-derived neurotrophic factor (BDNF) expression of adolescent C57BL/6J male mice after a 5-week two-bottle choice
Peripheral vascular reactivity and serum BDNF responses to aerobic training are impaired by the BDNF Val66Met polymorphism
Lemos Jr J R, et al.
Physiological Genomics, 48, 116-123 (2015)
The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function
Egan M F, et al.
Cell, 257-269 (2003)
Electroacupuncture at the Baihui acupoint alleviates cognitive impairment and exerts neuroprotective effects by modulating the expression and processing of brain-derived neurotrophic factor in APP/PS1 transgenic mice.
Lin R
Molecular Medicine Reports, 13(2), 1611-1617 (2016)
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