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Merck
CN

PICM03050

Millicell® Standing细胞培养池

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 13 mm, sterile

别名:

可渗透培养小室, 组织培养小室, 组织培养板, 细胞培养小室

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关于此项目

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202

主要文件

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产品名称

Millicell® Standing细胞培养池, pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 13 mm, sterile

material

polystyrene housing
transparent PTFE membrane

sterility

ethylene oxide treated
sterile

feature

hydrophilic

packaging

pack of 50 (individually blister packaged)

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

13 mm

diam.

30 mm

filtration area

4.2 cm2

size

6 wells

surface area

4.2 cm2

working volume

1.5 mL

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

Quality Level

400

color

transparent membrane, when wetted

matrix

Biopore™

pore size

0.4 μm

相关类别

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany

Application

  • 细胞附着
  • 细胞生长
  • 细胞分化
  • 免疫细胞化学

Features and Benefits

  • 促进细胞的卓越生长，并为细胞研究提供了绝佳机会。
  • 低蛋白吸附的亲水性PTFE膜非常适合活细胞观察和免疫荧光应用。

General description

凭借Millicell®立式插件，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。另外，Millicell®立式插件可用于从两侧研究单层细胞。

分析证书(COA)

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CB1 receptors and post-ischemic brain damage: Studies on the toxic and neuroprotective effects of cannabinoids in rat organotypic hippocampal slices.
Landucci, Elisa, et al.
Neuropharmacology, 60, 674-682 (2011)
Synapse elimination in olivo-cerebellar explants occurs during a critical period and leaves an indelible trace in Purkinje cells.
Letellier, Mathieu, et al.
Proceedings of the National Academy of Sciences of the USA, 106, 14102-14107 (2009)
Viktoria Melcher et al.
Acta neuropathologica, 139(5), 913-936 (2019-12-19)
Atypical teratoid/rhabdoid tumors (ATRT) are known for their heterogeneity concerning pathophysiology and outcome. However, predictive factors within distinct subgroups still need to be uncovered. Using multiplex immunofluorescent staining and single-cell RNA sequencing we unraveled distinct compositions of the immunological tumor
Daniela Buonvicino et al.
British journal of pharmacology, 177(14), 3342-3356 (2020-03-22)
Drugs able to counteract progressive multiple sclerosis (MS) represent a largely unmet therapeutic need. Even though the pathogenesis of disease evolution is still obscure, accumulating evidence indicates that mitochondrial dysfunction plays a causative role in neurodegeneration and axonopathy in progressive
Preparation of organotypic hippocampal slice cultures: interface method.
De Simoni, Anna and Yu, Lily M Y
Nature Protocols, 1, 1439-1445 (2006)
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实验方案

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

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