产品名称
抗脑源性神经营养因子抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... BDNF(627)
Analysis Note
对照
脑组织
脑组织
Application
免疫组化：1-10 μg/mL（请参阅建议的方案）。
免疫印迹：1-10 μg/mL。BDNF在标准缓冲液中不易提取。切开的组织应在10倍体积的100mM磷酸盐缓冲液中匀浆，该缓冲液包含1mM EDTA，2M盐酸胍（pH7.2）和三种蛋白酶抑制剂，10mM N-乙基马来酰亚胺，0.36 mM胃抑素和1mM PMSF。然后对匀浆进行超声处理，并在4°C下以46K x g离心30分钟。 BDNF迁移为18 kDa，同型二聚体约为27-30 kDa。
ELISA（单位点）：1-10 μg/mL
最佳工作稀释度必须由最终用户进行确定。
免疫印迹：1-10 μg/mL。BDNF在标准缓冲液中不易提取。切开的组织应在10倍体积的100mM磷酸盐缓冲液中匀浆，该缓冲液包含1mM EDTA，2M盐酸胍（pH7.2）和三种蛋白酶抑制剂，10mM N-乙基马来酰亚胺，0.36 mM胃抑素和1mM PMSF。然后对匀浆进行超声处理，并在4°C下以46K x g离心30分钟。 BDNF迁移为18 kDa，同型二聚体约为27-30 kDa。
ELISA（单位点）：1-10 μg/mL
最佳工作稀释度必须由最终用户进行确定。
抗脑源性神经营养性因子抗体是一种抗脑源性神经营养性因子抗体，用于ELISA、IH & WB。
研究子类别
神经化学 & 神经营养因子
神经炎症 & 疼痛
神经化学 & 神经营养因子
神经炎症 & 疼痛
研究类别
神经科学
神经科学
Biochem/physiol Actions
可识别脑源性神经营养因子（BDNF）。通过斑点印迹，与小鼠NGF，重组人NT3或神经营养蛋白4的交叉反应性小于0.1%。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
18 kDa（单体）和27-30 kDa（同型二聚体）
Immunogen
10个氨基酸的肽，对应于与卵清蛋白偶联的小鼠脑源性神经营养因子（BDNF）的氨基末端。序列为H2N-HSDPARRGEL-COOH。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
从PBS冻干的蛋白A纯化免疫球蛋白级分，不含防腐剂。用500 μL无菌去离子水复溶（1 mg/mL）。
形式：纯化
纯化的蛋白 A
Preparation Note
以未稀释的等分试样的形式在-20至-70°C下保存长达12个月。应避免反复冻/融循环。可以添加甘油（1:1）以获得额外的稳定性。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Ewa Badowska-Szalewska et al.
Acta neurobiologiae experimentalis, 77(1), 68-76 (2017-04-06)
The way hippocampal neurons function during stress in old age (critical times of life) is dependent on brain derived neurotrophin factor (BDNF). This study examined the influence of acute and chronic forced swim (FS) or high-light open field (HL‑OF) stimulation
Antonino Germanà et al.
International journal of molecular sciences, 21(16) (2020-08-19)
Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is involved in multiple and fundamental functions of the central and peripheral nervous systems including sensory organs. Despite recent advances in knowledge on the functional significance of BDNF and TrkB
Luciano Domenici et al.
PloS one, 9(12), e115579-e115579 (2014-12-24)
Vision loss in glaucoma is caused by progressive dysfunction of retinal ganglion cells (RGCs) and optic nerve atrophy. Here, we investigated the effectiveness of BDNF treatment to preserve vision in a glaucoma experimental model. As an established experimental model, we
Qiong Wu et al.
Journal of neuroscience research, 95(12), 2483-2492 (2017-07-13)
Currently antidepressants take several weeks to be effective, which is one of the main reasons why patients with depression quit therapy. In the present study, we examine the acute and subacute effects of soluble epoxide hydolase (sEH) inhibitor (sEHI), a
Ghiabe-Henri Guibinga et al.
PloS one, 9(5), e96575-e96575 (2014-05-09)
Lesch-Nyhan Syndrome (LNS) is a neurodevelopmental disorder caused by mutations in the gene encoding the purine metabolic enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT). This syndrome is characterized by an array of severe neurological impairments that in part originate from striatal dysfunctions. However
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