Volatile Fraction of Limoncello Using SPME on the SLB-5ms

技术

gas chromatography (GC): suitable
solid phase microextraction (SPME): suitable

试验参数

sample/matrix: Limoncello diluted 1:100 in water, then 5 mL of this solution transferred to a 10 mL crimped vial spiked with 100 μL of a 100 ppm solution of nonane (internal standard)
SPME fiber: 100 μm polydimethylsiloxane (57300-U)
extraction: headspace, with a pre-equilibration period of 10 min. at 40 °C, and then a fiber exposure of 30 min. at 40 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 ppm
desorption process: 250 °C for 5 min
column: SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven: 40 °C, 3 °C/min to 250 °C (2 min)
inj. temp.: 250 °C
detector: FID, 260 °C (sampling rate 80 ms)
carrier gas: helium, 35.0 cm/sec
injection: splitless
liner: 0.75 mm I.D. SPME (2633901)
sample: Limoncello liquor

适用性

application for SPME

应用

food and beverages