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主要文件

P1644

Sigma-Aldrich

Percoll®

pH 8.5-9.5 (20 °C)

别名:

Percoll密度梯度介质

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100 μG
¥4,220.99

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100 μG
¥4,220.99

About This Item

MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

¥4,220.99


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无菌性

aseptically filled

质量水平

表单

colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )

技术

cell culture | mammalian: suitable

颜色

clear colorless to faint yellow

pH值(酸碱度)

8.5-9.5 (20 °C)

应用

hematology
histology

储存温度

2-8°C

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此商品
PHR14167140925116
Quality Level

300

Quality Level

300

Quality Level

100

Quality Level

200

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

-

application(s)

-

form

powder

form

-

form

powder

form

-

pH

7.5-9 (25 °C, 29.4 g/L)

pH

-

pH

-

pH

7.5-9.0 (20 °C, 5%)

agency

meets USP testing specifications

agency

traceable to USP 1613859

agency

USP/NF, tested according to Ph. Eur.

agency

-

一般描述

Percoll®是一种用于细胞、病毒和亚细胞颗粒密度梯度离心的经典培养基。Percoll由直径为15-30 nm(在水中为23% w/w)并涂覆有聚乙烯吡咯烷酮(PVP)的胶体二氧化硅颗粒组成。PVP涂层使产品完全无毒,非常适合与生物材料一起使用。

应用

Percoll®已用于在精子准备中以研究牛胚胎的能量需求。[1]
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成,这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用于细胞分离和细胞器分离。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成,这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用来建立无毒低粘度,低渗透压密度梯度,可用于分离细胞,细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的,无毒,并且很容易从纯化的材料中去除。

法律信息

Percoll is a registered trademark of Cytiva

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

  • 技术规格说明书

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    1. Can you help me calculate gradients for Percoll®, Product P1644?

      The product information sheet (under Documents, above) has an equation for calculating density of a given solution.

    2. When using Product P1644, Percoll®, what density should I use for my particular system?

      The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.

    3. Is there a "best" salt solution to use with Product P1644, Percoll®?

      Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested.  Sucrose is included to prevent aggregation.  You can find detailed preparation instructions on the product information sheet (under Documents, above) for this product.

    4. Can Percoll®, Product P1644, be autoclaved?

      Percoll can be autoclaved, but this must be done without any salts or sucrose present.

    5. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    6. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    7. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    8. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    9. What is the difference between Percoll® Products P4937 and P1644?

      Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.

    10. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Development of bovine embryos in vitro as affected by energy substrates.
    Rosenkrans Jr C F, et al.
    Biology of Reproduction, 49(3), 459-462 (1993)
    T Hirano et al.
    The Journal of cell biology, 106(4), 1171-1183 (1988-04-01)
    A temperature-sensitive mutant nuc2-663 of the fission yeast Schizosaccharomyces pombe specifically blocks mitotic spindle elongation at restrictive temperature so that nuclei in arrested cells contain a short uniform spindle (approximately 3-micron long), which runs through a metaphase plate-like structure consisting
    Guilherme D Melo et al.
    Scientific reports, 7(1), 8454-8454 (2017-08-18)
    Visceral leishmaniasis (VL) is a systemic disease with multifaceted clinical manifestations, including neurological signs, however, the involvement of the nervous system during VL is underestimated. Accordingly, we investigated both brain infection and inflammation in a mouse model of VL. Using
    Rajasree Sreedharan et al.
    Kidney international, 86(3), 515-524 (2014-05-09)
    Inducible heat shock proteins (HSPs), regulated by heat shock factor-1 (HSF-1), protect against renal cell injury in vitro. To determine whether HSPs ameliorate ischemic renal injury in vivo, HSF-1 functional knockout mice (HSF-KO) were compared with wild-type mice following bilateral
    R Hovius et al.
    Biochimica et biophysica acta, 1021(2), 217-226 (1990-01-29)
    Rat liver mitochondria were isolated by a combination of differential and Percoll gradient centrifugation, resulting in a highly pure and intact preparation, as assessed by marker enzyme analysis, latency of cytochrome-c oxidase, respiratory control index and electron microscopy. Two different

    商品

    Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

    实验方案

    为了配置Percoll梯度液,必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。

    To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

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