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Merck
CN

P7828

Sigma-Aldrich

Percoll®

suitable for plant cell culture

别名:

density gradient medium

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About This Item

MDL编号:
UNSPSC代码:
10171502
NACRES:
NA.72

无菌性

aseptically filled

质量水平

技术

cell culture | plant: suitable

pH值(酸碱度)

8.9 (20 °C)

应用

agriculture

储存温度

2-8°C

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应用

Percoll ® 已用于密度梯度离心分离绿藻 、蜕膜基质细胞 和公鸡精子的不同种群的完整叶绿体。
Percoll 用于植物研究中的原生质体和细胞器(叶绿体、线粒体)分离。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成,这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用来建立无毒低粘度,低渗透压密度梯度,可用于分离细胞,细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的,无毒,并且很容易从纯化的材料中去除。

法律信息

Percoll is a registered trademark of Cytiva

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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Guangkun Yin et al.
Plant & cell physiology, 50(7), 1305-1318 (2009-06-13)
The development of mitochondria during seed germination is essential for plant growth. However, the developmental process is still poorly understood. Temperature plays a key role in soybean germination, and in this study we characterized the mitochondrial ultrastructure and proteome after
A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii
Mason CB, et al.
Nature Protocols, 1(5), 2227-2227 (2006)
Xiang-Jie Yin et al.
Clinical proteomics, 18(1), 9-9 (2021-02-24)
Pregnancy is a complicated physiological process. The multifaceted regulation of maternal-fetal interface is of great importance for maintaining normal pregnancy and avoiding fetal rejection and secondary abortion. Previous studies have focused on the clinical features or pathological biomarkers of fetal
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Ahammad MU, et al.
Journal of Visualized Experiments, 145(141), e58833-e58833 (2018)
Teresa D'Amico Oblak et al.
The Analyst, 134(1), 188-193 (2008-12-17)
A simple poly(dimethylsiloxane) (PDMS) microchip was employed to establish a relationship between red blood cell (RBC) antioxidant status and the ability of RBCs to interact with metal-activated C-peptide, a bio-active peptide reported to reduce some complications often associated with diabetes.

实验方案

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

为了配置Percoll梯度液,必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。

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