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P7828

Percoll^®

Name: Percoll<SUP>®</SUP>
Brand: SIGMA

suitable for plant cell culture

别名:

density gradient medium

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About This Item

MDL编号:

MFCD00131830

UNSPSC代码:

10171502

NACRES:

NA.72

产品P7828监管中，请联系销售询价或购买联系客户支持

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此商品	FD2000S	FD40S	FD250S
52471 Fluorescein isothiocyanate–dextran	FD2000S Fluorescein isothiocyanate–dextran	FD40S Fluorescein isothiocyanate–dextran	FD250S Fluorescein isothiocyanate–dextran
biological source synthetic	biological source synthetic (organic)	biological source synthetic (organic)	biological source bacterial (Leuconostoc mesenteroides)
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 200
form powder or crystals	form powder	form powder	form powder
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate
mol wt average mol wt 2,000,000	mol wt average mol wt 2,000,000	mol wt average mol wt 40,000	mol wt average mol wt 250,000
extent of labeling 0.001-0.020 mol FITC per mol glucose (substitution)	extent of labeling 0.003-0.020 mol FITC per mol glucose	extent of labeling 0.003-0.020 mol FITC per mol glucose	extent of labeling 0.003-0.020 mol FITC per mol glucose

应用

Percoll ^® 已用于密度梯度离心分离绿藻 [1]、蜕膜基质细胞 [2] 和公鸡精子的不同种群的完整叶绿体。[3]

Percoll 用于植物研究中的原生质体和细胞器（叶绿体、线粒体）分离。

Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成，这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。它被用于细胞分离和细胞器分离。

Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成，这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。它被用来建立无毒低粘度，低渗透压密度梯度，可用于分离细胞，细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的，无毒，并且很容易从纯化的材料中去除。

法律信息

Percoll is a registered trademark of Cytiva

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

历史批次信息供参考:

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Mitochondrial damage in the soybean seed axis during imbibition at chilling temperatures.

Guangkun Yin et al.

Plant & cell physiology, 50(7), 1305-1318 (2009-06-13)

The development of mitochondria during seed germination is essential for plant growth. However, the developmental process is still poorly understood. Temperature plays a key role in soybean germination, and in this study we characterized the mitochondrial ultrastructure and proteome after

A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii

Mason CB, et al.

Nature Protocols, 1(5), 2227-2227 (2006)

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Ahammad MU, et al.

Journal of Visualized Experiments, 145(141), e58833-e58833 (2018)

Proteomic analysis of decidua in patients with recurrent pregnancy loss (RPL) reveals mitochondrial oxidative stress dysfunction.

Xiang-Jie Yin et al.

Clinical proteomics, 18(1), 9-9 (2021-02-24)

Pregnancy is a complicated physiological process. The multifaceted regulation of maternal-fetal interface is of great importance for maintaining normal pregnancy and avoiding fetal rejection and secondary abortion. Previous studies have focused on the clinical features or pathological biomarkers of fetal

Morphological, molecular, and phylogenetic characterization of Nosema ceranae, a microsporidian parasite isolated from the European honey bee, Apis mellifera.

Yanping P Chen et al.

The Journal of eukaryotic microbiology, 56(2), 142-147 (2009-05-22)

Nosema ceranae, a microsporidian parasite originally described from Apis cerana, has been found to infect Apis melllifera and is highly pathogenic to its new host. In the present study, data on the ultrastructure of N. ceranae, presence of N. ceranae-specific

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实验方案

如何制作和使用Percoll梯度

为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

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Percoll^®