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Merck
CN
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文件

P4937

Sigma-Aldrich

Percoll®

pH 8.5-9.5 (25 °C), suitable for cell culture

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MDL编号:
MFCD00131830
UNSPSC代码:
12161902
NACRES:
NA.75

无菌性

aseptically filled

质量水平

200

技术

cell culture | mammalian: suitable
single cell analysis: suitable

pH值（酸碱度）

8.5-9.5 (25 °C)

密度

1.13 g/mL±0.005 g/mL at 25 °C (lit.)

储存温度

2-8°C

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一般描述

Percoll®是一种用于细胞、病毒和亚细胞颗粒密度梯度离心的经典培养基。它由直径为15-30 nm（在水中为23％ w/w）并涂覆有聚乙烯吡咯烷酮（PVP）的胶体二氧化硅颗粒组成。PVP涂层使产品完全无毒，非常适合与生物材料一起使用。由于其颗粒大小的不均匀性，沉积以不同的速率发生，自发地在1.0-1.3 g/ml范围内产生非常光滑的等距梯度。

应用

Percoll®已用于以下材料的离心分离：
  • 新生大鼠心室心肌细胞
  • 血液样本中的白细胞
  • 小管周围肌样（PM）细胞
适用于制备单细胞悬浮液以进行测序。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成，这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用来建立无毒低粘度，低渗透压密度梯度，可用于分离细胞，细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的，无毒，并且很容易从纯化的材料中去除。

特点和优势

  • 低渗透压允许对生理条件进行精确调整，而不会受到介质的明显干扰。
  • 与活细胞和病毒兼容，并允许分离和恢复完整的，完全活跃的系统。
  • 对生物膜不渗透，在离心过程中不改变颗粒的浮力密度。
  • 在离心过程中自发形成的梯度允许在离心管中混合大量样品。
  • 低粘度导致快速形成梯度和颗粒分离。
  • 大多数沉降系数大于60S的生物颗粒可以在Percoll梯度中成功分离。

法律信息

Percoll is a registered trademark of Cytiva

相关产品

产品编号
说明
价格

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Can you help me calculate gradients for Percoll®, Product P1644?

    The product information sheet (under Documents, above) has an equation for calculating density of a given solution.

  2. When using Product P1644, Percoll®, what density should I use for my particular system?

    The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.

  3. Is there a "best" salt solution to use with Product P1644, Percoll®?

    Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested.  Sucrose is included to prevent aggregation.  You can find detailed preparation instructions on the product information sheet (under Documents, above) for this product.

  4. Can Percoll®, Product P1644, be autoclaved?

    Percoll can be autoclaved, but this must be done without any salts or sucrose present.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. What is the difference between Percoll® Products P4937 and P1644?

    Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

如何制作和使用Percoll梯度

为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。

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