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Key Documents

D1556

Sigma-Aldrich

OptiPrep Density Gradient Medium

used for cell and subcellular organelle isolation

Synonym(s):

Iodixanol solution

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25 μG
CN¥1,455.13

CN¥1,455.13


Estimated to ship onMay 13, 2025Details


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25 μG
CN¥1,455.13

About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

CN¥1,455.13


Estimated to ship onMay 13, 2025Details


Request a Bulk Order

sterility

sterile

form

liquid

density

1.319-1.321 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

SMILES string

CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I

InChI

1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)

InChI key

NBQNWMBBSKPBAY-UHFFFAOYSA-N

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format

neat

format

-

format

-

format

-

CofA

current certificate can be downloaded

CofA

-

CofA

current certificate can be downloaded

CofA

-

packaging

ampule of 1000 mg

packaging

-

packaging

pkg of 1 g

packaging

-

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

-

technique(s)

-

refractive index

n20/D 1.4743 (lit.)

refractive index

n20/D 1.4743 (lit.)

refractive index

n20/D 1.4743 (lit.)

refractive index

n20/D 1.4743 (lit.)

General description

OptiPrep Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles.[1]

Application

OptiPrep has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells.[1] It has also been used for the following applications:

  • to investigate the efficiency of single-cell encapsulation.[1]
  • the gradient-based isolation of senescent cancer cells.[2]
  • isolation of non-enveloped virion isolation from bronchoalveolar lavage.[3]
  • isolation of lipoproteins and macromolecules
  • purification of virions
  • purification of neuron cells

Features and Benefits

OptiPrep has numerous biocompatible properties that make it ideal for use.
  • It is biologically inert and does not interfere with assays for marker enzymes.[1]
  • It is non-ionic, non-toxic, and has low osmotic pressure.
  • It forms spontaneous gradients that are stabler and easier to prepare than sucrose.[2]

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


  • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?

      Percoll and Histopaque must be removed prior to use through washing.  If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells.  In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.

    4. What is the appearance of Product D1556, OptiPrep?

      The solution may be clear to slightly yellow.

    5. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    6. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    7. How do I store solutions of Product D1556, OptiPrep Density Gradient Medium?

      After diluting Product D1556, OptiPrep Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.

    8. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Tanner C Fadero et al.
    eLife, 6 (2017-09-05)
    Iodixanol provides an easy and affordable solution to a problem that has limited resolution and brightness when imaging living samples.
    Isolation of senescent cells by iodixanol (OptiPrep) density gradient-based separation
    Kristina Kovacovicova, Manlio Vinciguerra
    Cell Proliferation, 52 (2019)
    WU Polyomavirus in Respiratory Epithelial Cells from Lung Transplant Patient with Job Syndrome
    Erica A. S, et al.
    Emerging Infectious Diseases, 21(1), 103-106 (2015)
    Eloise Hudry et al.
    Molecular therapy : the journal of the American Society of Gene Therapy, 18(1), 44-53 (2009-08-06)
    The development of Alzheimer's disease (AD) is closely connected with cholesterol metabolism. Cholesterol increases the production and deposition of amyloid-beta (Abeta) peptides that result in the formation of amyloid plaques, a hallmark of the pathology. In the brain, cholesterol is
    Lysosome-Related Effector Vesicles in T Lymphocytes and NK Cells
    Lettau, et al.
    Scandinavian Journal of Immunology, 82 (2015)

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