Skip to Content
Merck
CN
All Photos(1)

Key Documents

View All Documentation

P7828

Sigma-Aldrich

Percoll®

suitable for plant cell culture

Synonym(s):

density gradient medium

Sign Into View Organizational & Contract Pricing

Select a Size

25 G
CN¥620.51
100 G
CN¥1,541.57
500 G
CN¥2,269.88

CN¥620.51

Estimated to ship onApril 07, 2025Details

Request a Bulk Order
All Photos(1)

Select a Size

Change View
25 G
CN¥620.51
100 G
CN¥1,541.57
500 G
CN¥2,269.88

About This Item

MDL number:
MFCD00131830
UNSPSC Code:
10171502
NACRES:
NA.72

CN¥620.51

Estimated to ship onApril 07, 2025Details

Request a Bulk Order

sterility

aseptically filled

Quality Level

200

form

colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))

technique(s)

cell culture | plant: suitable

pH

8.9 (20 °C)

application(s)

agriculture

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
K1750K1128O4126
α-Ketoglutaric acid 99.0-101.0% (T)

75890

α-Ketoglutaric acid

α-Ketoglutaric acid ≥98.5% (NaOH, titration)

K1750

α-Ketoglutaric acid

α-Ketoglutaric acid BioReagent, suitable for cell culture, suitable for insect cell culture

K1128

α-Ketoglutaric acid

Oxaloacetic acid ≥97% (HPLC)

O4126

Oxaloacetic acid

solubility

H2O: 1 g/10 mL, clear to slightly hazy, colorless to almost colorless

solubility

water: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

solubility

water: 100 mg/mL, clear to slightly hazy, colorless to almost colorless

solubility

H2O: 100 mg/mL, clear to slightly hazy, colorless to light yellow

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

mp

114-118 °C

mp

-

mp

-

mp

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

form

crystals

form

powder

form

powder or crystals

form

powder

Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll is used in plant research for protoplast and organelle (chloroplast, mitochondria) separation.
Percoll® has been used in the density gradient centrifugation isolation of intact chloroplasts from green alga[1], decidual stromal cells[2] and distinct populations of rooster sperm.[3]

Legal Information

Percoll is a registered trademark of Cytiva

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000427948
    0000427948
    0000395058
    0000366400
    0000361138

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Xiang-Jie Yin et al.
    Clinical proteomics, 18(1), 9-9 (2021-02-24)
    Pregnancy is a complicated physiological process. The multifaceted regulation of maternal-fetal interface is of great importance for maintaining normal pregnancy and avoiding fetal rejection and secondary abortion. Previous studies have focused on the clinical features or pathological biomarkers of fetal
    Sperm Collection of Differential Quality Using Density Gradient Centrifugation
    Ahammad MU, et al.
    Journal of Visualized Experiments, 145(141), e58833-e58833 (2018)
    Guangkun Yin et al.
    Plant & cell physiology, 50(7), 1305-1318 (2009-06-13)
    The development of mitochondria during seed germination is essential for plant growth. However, the developmental process is still poorly understood. Temperature plays a key role in soybean germination, and in this study we characterized the mitochondrial ultrastructure and proteome after
    A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii
    Mason CB, et al.
    Nature Protocols, 1(5), 2227-2227 (2006)
    The decidual stromal cells-secreted CCL2 induces and maintains decidual leukocytes into Th2 bias in human early pregnancy
    He YY, et al.
    Clinical Immunology (Orlando, Fla.), 145(2), 161-173 (2012)
    View All Related Papers

    Protocols

    How to Make and Use Percoll Gradients

    To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service