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D1556

Sigma-Aldrich

OptiPrep™密度梯度培养基

used for cell and subcellular organelle isolation

别名:

碘克沙醇 溶液

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About This Item

CAS号:
92339-11-2
MDL编号:
MFCD00867965
UNSPSC代码:
12352207
PubChem化学物质编号:
329798703
NACRES:
NA.75

质量水平

200

无菌性

sterile

表单

liquid

密度

1.319-1.321 g/mL

应用

hematology
histology

SMILES字符串

CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I

InChI

1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)

InChI key

NBQNWMBBSKPBAY-UHFFFAOYSA-N

相关类别

一般描述

OptiPrep™密度梯度培养基，也称为碘二醇，是一种渗透压低、非离子型碘化密度梯度培养基。它是一种无菌且经内毒素测试的60%（w/v）碘乙醇水溶液，用于体外分离生物颗粒。它可以用于细胞和亚细胞细胞器组分分离。

应用

OptiPrep™可用于分离分子、细胞和亚细胞器，包括DNA、细胞因子、溶酶体、内质网、过氧化物酶体、T淋巴细胞和自然杀伤细胞。它还被用于以下应用领域：

  • 研究单个细胞封装的效率。
  • 基于梯度的分离衰老癌细胞。
  • 从支气管肺泡灌洗液中分离非包膜病毒颗粒。
  • 分离脂蛋白和大分子
  • 纯化病毒颗粒
  • 纯化神经元细胞

特点和优势

OptiPrep™具有许多生物相容性特性，使其成为理想的使用选择。
  • 它在生物学上是惰性的，不会干扰标志酶的测定。
  • 它是非离子型，无毒且渗透压低。
  • 它能够形成自发梯度，比蔗糖稳定、更容易制备。

其他说明

60% (w/v) 碘克沙醇水溶液（无菌）。

法律信息

OptiPrep is a trademark of Serumwerk Bernburg AG

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
MKCV5254
MKCW0884
MKCX0606
MKCW4446
MKCW8861

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?

    Percoll and Histopaque must be removed prior to use through washing.  If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells.  In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.

  4. What is the appearance of Product D1556, OptiPrep?

    The solution may be clear to slightly yellow.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. How do I store solutions of Product D1556, OptiPrep™ Density Gradient Medium?

    After diluting Product D1556, OptiPrep™ Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Eloise Hudry et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 18(1), 44-53 (2009-08-06)
The development of Alzheimer's disease (AD) is closely connected with cholesterol metabolism. Cholesterol increases the production and deposition of amyloid-beta (Abeta) peptides that result in the formation of amyloid plaques, a hallmark of the pathology. In the brain, cholesterol is
Chapter 19: Isolation of Subcellular Organelles and Structures
Uwe Michelsen, Jorg von Hagen
Methods in Enzymology, 463 (2009)
Lysosome-Related Effector Vesicles in T Lymphocytes and NK Cells
Lettau, et al.
Scandinavian Journal of Immunology, 82 (2015)
Tanner C Fadero et al.
eLife, 6 (2017-09-05)
Iodixanol provides an easy and affordable solution to a problem that has limited resolution and brightness when imaging living samples.
Isolation of senescent cells by iodixanol (OptiPrep) density gradient-based separation
Kristina Kovacovicova, Manlio Vinciguerra
Cell Proliferation, 52 (2019)
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