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10771

Sigma-Aldrich

Histopaque®-1077

sterile-filtered, density: 1.077

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

400

表单

liquid

保质期

Expiry date on the label.

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

密度

1.077

应用

hematology
histology

储存温度

2-8°C

相关类别

一般描述

Histopaque-1077 是一种无菌、经过内毒素测试的即用型培养基。Histopaque组合液包括聚蔗糖和泛影酸钠，密度经调节后为1.077g/mL。这种即用型试剂有助于从小体积的全血或骨髓中进行活性淋巴细胞和其他单个核细胞的快速恢复。

应用

Histopaque-1077用于：
  • 人淋巴细胞抗原(HLA)分型
  • T、B和‘裸’淋巴细胞计数前的起始分离。
  • 制备用于细胞培养和细胞毒性试验的纯淋巴细胞悬液。
  • 分离和纯化外周血单个核细胞(PBMC)和骨髓单个核细胞(BMMC)
  • 分离人类淋巴细胞
  • 分离全血中乳癌循环肿瘤细胞(CTC)
  • Histopaque-1077适合与Histopaque-1119一起使用，因为其有助于从少量全血中快速恢复活性单个核细胞和粒细胞

特点和优势

Histopaque®-1077 有许多优势，比如：
  • 可使活细胞极佳恢复
  • 允许选择分离不同种类的血细胞
  • 防止细胞变形
  • 保持细胞活力
  • 最小化外来细胞干扰
  • 各批次性能可重复
  • 经过过滤杀菌和内毒素检测
  • 在 2-8°C 避光保护条件下可稳定保存至少2年

原理

将抗凝血铺在Histopaque-1077液面上。离心时，红细胞被聚蔗糖聚集并迅速沉淀。粒细胞变得略微高渗，它们的沉降速率增加，在ACCUSIN管底部形成沉淀。淋巴细胞和其他单个核细胞留在血浆/Histopaque-1077分界面。

法律信息

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

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象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM1878
RNBM0545
RNBM0063
RNBL7683
RNBM0064

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  1. Is Histopaque®, Product 10771, the same as Ficoll® solution, Product F5415?

    Ficoll-Hypaque is a mixture of Ficoll (polysucrose) and Hypaque (sodium diatrizoate).  It does not specify a density.  Product No. 10771 is a mixture of polysucrose and sodium diatrizoate at a density of 1.077 g/ml.

  2. Should I use Histopaque® 10771 or 10831 for isolation of lymphocytes?

    The different product numbers reflect the density of the solution.  Product No. 10771 is to be used to isolate human mononuclear cells.  Product No. 10831 is to be used for the isolation of mononuclear cells from mice, rats and other mammals.

  3. What is the difference between Histopaque® Product No. 10771 and H8889?

    Product 10771, Histopaque®-1077, is not cell culture tested and Product H8889, Histopaque®-1077 Hybri-Max™, is cell culture tested.Both Products are sterile, endotoxin tested and intended for Research Use Only. 

  4. What is the osmolarity of the Product 10771, Histopaque®-1077 solution? 

    The osmolarity is a property that is tested for each lot of material; the range of acceptable values is 280.0 - 300.0 mOs/kg.  The lot specific value is given on the Certificate of Analysis.  Certificates of Analysis can be viewed on our website by entering the lot number on our Advanced Search Page.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Erin E Thacker et al.
Vaccine, 27(50), 7116-7124 (2009-09-30)
Targeting viral vectors encoding tumor-associated antigens to dendritic cells (DCs) in vivo is likely to enhance the effectiveness of immunotherapeutic cancer vaccines. We have previously shown that genetic modification of adenovirus (Ad) 5 to incorporate CD40 ligand (CD40L) rather than
Ana C Leal et al.
Scientific reports, 7(1), 6438-6438 (2017-07-27)
Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release of tumor-derived extracellular vesicles and the activation of host vascular cells. It was proposed that neutrophil extracellular
Evaluation of respiration with clark type electrode in isolated mitochondria and permeabilized animal cells.
Silva AM and Oliveira PJ
Methods in Molecular Biology, 810, 7-24 (2012)
Romain A Colas et al.
Journal of immunology (Baltimore, Md. : 1950), 197(11), 4444-4452 (2016-11-02)
Maresin 1 (MaR1) is an immunoresolvent that governs resolution of acute inflammation, and its local metabolism in the context of infectious inflammation is of interest. In this study, we investigated the MaR1 metabolome in infectious exudates and its bioactions in
Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
Boyle JJ et al
The American Journal of Pathology, 174(3), 1097-1108 (2009)
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