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此商品 | A9045 | A0701 | A4718 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| gel strength ≥200 g/cm2 (1% gel) | gel strength ≥200 g/cm2 (1% gel) | gel strength ≥250 g/cm2 (1% gel) | gel strength - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| transition temp congealing temperature 26-30 °C | transition temp congealing temperature 26-30 °C | transition temp gel point 26 °C ±2 °C (1.5% gel) | transition temp gel point ≤35 °C (3% gel) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable |
一般描述
Percoll® is a well-referenced media for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll® is composed of colloidal silica coated with polyvinylpyrrolidone (PVP).
Percoll® is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 × g). The medium is available in easy-to-open, resealable 250 mL and 1 l bottles.
Percoll® consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is present at only 1% to 2%. Percoll® is non-toxic, almost chemically inert and does not adhere to membranes. Percoll® gradients can be formed within the density range of 1.0 to 1.3 g/mL, and are iso-osmotic throughout.
Percoll® can be stored unopened at room temperature for five years. At -20° C, it can only be stored for up to six months. If stored at -20° C, gradients form upon thawing, necessitating a mixing of the bottle before use. Preformed gradients can be stored for weeks without a change in gradient shape, provided that the gradient remains unfrozen. Percoll® can be buffered within the pH range 5.5 to 10.0 without any changes in properties. If the pH is dropped below 5.5, gelling may occur. Gelling can also be caused by the presence of divalent cations, an effect which is exacerbated by elevated temperatures.
Percoll® is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 × g). The medium is available in easy-to-open, resealable 250 mL and 1 l bottles.
Percoll® consists of silica particles (15 to 30 nm diameter) coated with non-dialyzable polyvinylpyrrolidone (PVP). Free PVP is present at only 1% to 2%. Percoll® is non-toxic, almost chemically inert and does not adhere to membranes. Percoll® gradients can be formed within the density range of 1.0 to 1.3 g/mL, and are iso-osmotic throughout.
Percoll® can be stored unopened at room temperature for five years. At -20° C, it can only be stored for up to six months. If stored at -20° C, gradients form upon thawing, necessitating a mixing of the bottle before use. Preformed gradients can be stored for weeks without a change in gradient shape, provided that the gradient remains unfrozen. Percoll® can be buffered within the pH range 5.5 to 10.0 without any changes in properties. If the pH is dropped below 5.5, gelling may occur. Gelling can also be caused by the presence of divalent cations, an effect which is exacerbated by elevated temperatures.
应用
Used in balanced salt solutions, physiological saline or 0.25 M sucrose.
特点和优势
- For separation of cells, subcellular particles and larger viruses (down to ~ 70S) under gentle conditions which preserve viability and morphological integrity.
- Non-toxic to cells.
- Adjustable to physiological ionic strength and pH.
- Gradients can either be preformed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor.
- Gradients are iso-osmotic throughout and cover a range of densities up to 1.3 g/mL.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Percoll is a registered trademark of Cytiva
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
实验方案
为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
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