生物来源
bovine pancreas
质量水平
形式
lyophilized
包装
pkg of 100 mg
制造商/商品名称
Roche
技术
cell culture | mammalian: suitable
储存温度
2-8°C
一般描述
DNase I是一种可降解DNA的双链特异性内切核酸酶。牛胰腺脱氧核糖核酸酶I（DNase I）是DNA小沟相互作用的核酸酶，特异性相对较低。该蛋白质由两个中心β折叠构成，而每个折叠由六个β-链所组成。该结构被大量的环以及α-螺旋区域包围。该酶与核酸外切酶III具有结构相似性。DNase I是研究得最透彻的哺乳动物来源的内切酶之一。
应用
DNase I 与胶原酶一起已被用于组织解离。
生化/生理作用
在组织解离过程中，部分细胞被裂解并导致DNA的释放。单分子DNA可能会导致细胞结团。向解离缓冲液中加入DNase I会导致该细胞外DNA的降解，因而避免了因意外结团造成的细胞损失。
特点和优势
- 冻干的
- 且冻干前经 0.2 μm 孔径膜过滤
- 糖蛋白和双链特异性内切酶
单位定义
一个单位表示在测定条件下导致吸光度以0.001每分钟的速率而增加的酶活力。
制备说明
激活剂：二价金属离子 (Ca2+, Mg2+)
工作浓度：0.01 至 1 mg/ml
注意：必须针对每种细胞类型单独确定工作浓度。为实现最佳活性，酶须添加 5 mM Mg2+。
工作浓度：0.01 至 1 mg/ml
注意：必须针对每种细胞类型单独确定工作浓度。为实现最佳活性，酶须添加 5 mM Mg2+。
重悬
用无菌的双蒸水对冻干酶进行重悬（10 mg/ml）；用PBS（磷酸盐缓冲液）、HBSS（Hank′s 平衡盐溶液）或培养基进行进一步的稀释。
其他说明
仅用于生命科学研究。不用于诊断操作。
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
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S Cal et al.
The EMBO journal, 17(23), 7128-7138 (1998-12-08)
Bovine pancreatic deoxyribonuclease I (DNase I) is a nuclease of relatively low specificity which interacts with DNA in the minor groove. No contacts are made between the protein and the major groove of the nucleic acid. DNase I is structurally
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