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应用
神经氨酸酶是一种重要的去糖基化酶，能够通过水解 α (2→6)、α (2→3)、α (2→8) 和 α (2→9) 键（按给定的顺序亲和力）裂解所有非还原的不分枝 N-乙酰神经氨酸和 N-羟乙酰神经氨酸残基。使用高浓度的酶和延长孵育时间也可裂解分支的唾液酸。脱唾液酸糖蛋白可通过用各种外切糖苷酶处理进一步表征，导致部分或完全 O-脱糖基化。SDS-PAGE 和 MALDI-TOF MS 通常用于纯化、结构分析和测序过程。这些技术还可去除糖蛋白的异质性和电荷。
包装
提供 5× 浓缩液反应缓冲液。
质量
专门配制了缓冲液，以确保最大的酶稳定性和试验灵敏度。
单位定义
在 37°C、pH 值为 5.5 的条件下，1 个单元每分钟从 2-（4-甲基伞形酮）α-D-N-乙酰神经氨酸中释放 1 nmol 4-甲基伞形酮。
外形
冻干粉末
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说明
价格
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Hongyun Li et al.
PloS one, 11(2), e0148238-e0148238 (2016-02-02)
Recent studies have shown that cerebral apoD levels increase with age and in Alzheimer's disease (AD). In addition, loss of cerebral apoD in the mouse increases sensitivity to lipid peroxidation and accelerates AD pathology. Very little data are available, however
Natallia Makarava et al.
Acta neuropathologica communications, 6(1), 92-92 (2018-09-14)
Last decade witnessed an enormous progress in generating authentic infectious prions or PrPSc in vitro using recombinant prion protein (rPrP). Previous work established that rPrP that lacks posttranslational modification is able to support replication of highly infectious PrPSc with assistance
Johan Nordholm et al.
The Journal of biological chemistry, 288(15), 10652-10660 (2013-03-01)
Interactions that facilitate transmembrane domain (TMD) dimerization have been identified mainly using synthetic TMDs. Here, we investigated how inherent properties within natural TMDs modulate their interaction strength by exploiting the sequence variation in the nine neuraminidase subtypes (N1-N9) and the
Audu J Natala et al.
Journal of medical entomology, 50(1), 85-93 (2013-02-23)
Amblyomma variegatum F. are obligate hematophagous ectoparasites of livestock that serve as the vectors of Ehrlichia ruminantium (formerly known as Cowdria ruminantium), the causative agent of heartwater disease. In the light of the fact that they are blood-feeding, their salivary
Dominic Meusch et al.
Nature, 508(7494), 61-65 (2014-02-28)
Tripartite Tc toxin complexes of bacterial pathogens perforate the host membrane and translocate toxic enzymes into the host cell, including in humans. The underlying mechanism is complex but poorly understood. Here we report the first, to our knowledge, high-resolution structures
商品
Glycan Sequencing Using Exoglycosidases
Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
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