应用
神经氨酸酶是一种重要的去糖基化酶,能够通过水解 α (2→6)、α (2→3)、α (2→8) 和 α (2→9) 键(按给定的顺序亲和力)裂解所有非还原的不分枝 N-乙酰神经氨酸和 N-羟乙酰神经氨酸残基。使用高浓度的酶和延长孵育时间也可裂解分支的唾液酸。脱唾液酸糖蛋白可通过用各种外切糖苷酶处理进一步表征,导致部分或完全 O-脱糖基化。SDS-PAGE 和 MALDI-TOF MS 通常用于纯化、结构分析和测序过程。这些技术还可去除糖蛋白的异质性和电荷。
包装
提供 5× 浓缩液反应缓冲液。
质量
专门配制了缓冲液,以确保最大的酶稳定性和试验灵敏度。
单位定义
在 37°C、pH 值为 5.5 的条件下,1 个单元每分钟从 2-(4-甲基伞形酮)α-D-N-乙酰神经氨酸中释放 1 nmol 4-甲基伞形酮。
外形
冻干粉末
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产品编号
说明
价格
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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商品
Glycan Sequencing Using Exoglycosidases
Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
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