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安全信息

G4510

Millipore

谷胱甘肽−琼脂糖

lyophilized powder

别名:

谷胱甘肽-琼脂糖树脂, GSH-琼脂糖, S-连接谷胱甘肽琼脂糖

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About This Item

MDL编号:
MFCD00131200
UNSPSC代码:
41106500
NACRES:
NA.56

表单

lyophilized powder

质量水平

200

分析物化学类别

proteins (GST)

标记范围

≥10 μmol per mL gel

技术

immunoprecipitation (IP): suitable
protein purification: suitable

基质

4% cross-linked beaded agarose

基质活化

epoxy

基质附着

sulfur

基质隔离区

12 atoms (10 carbon)

储存温度

−20°C

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相关类别

一般描述

谷胱甘肽硫转移酶(GST)是由天然真核细胞生产的26 KDa分子量蛋白。它属于II相解毒酶家族的一员。谷胱甘肽-琼脂糖树脂由谷胱甘肽通过硫与环氧活化的4%交联琼脂糖珠相连形成12个原子（10碳）的间隔臂。本品为冻干粉末，添加了乳糖稳定剂。

应用

谷胱甘肽琼脂糖树脂用于：
  • 纯化谷胱甘肽硫转移酶(GST)融合蛋白，生成多克隆抗体M4P
  • 通过孵育上清液组分，纯化全长p53蛋白(aa 1-393)及其DNA结合域(aa 94-288)
  • 融合蛋白的亲和纯化

利用谷胱甘肽-琼脂糖亲和层析技术，可以快速、温和、非变性和高度选择性地纯化含有谷胱甘肽结合序列的蛋白质，如谷胱甘肽S-转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I。

生化/生理作用

谷胱甘肽硫转移酶(GST)属于多功能解毒同工酶超家族成员，在细胞信号传导中起重要作用。由于其特定同工酶在多种肿瘤中过表达，并且可能参与神经退行性疾病、多发性硬化症和哮喘等其他疾病的发生发展，GST成为前景可期的治疗靶标。

特点和优势

  • 提供快速、温和、非变性、高度选择性的谷胱甘肽结合酶纯化。
  • 可在-20 °C恰当干燥保存2年。
  • 载量：每mL树脂可结合5-10 mg谷胱甘肽S-转移酶

数量

1g粉末可溶胀至 ≥10 ml凝胶

外形

用乳糖稳定化的冻干粉

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000379659
0000371606
0000371595
0000275592
0000328867

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How much Product G4510, Glutathione-Agarose, do I receive and how do I swell my powder?

    This material is sold by the final volume of gel produced after swelling of the powder.  One gram of powder swells to approximately 14 mL of gel. (Approximately 70 mg of powder swells to 1 mL of gel.)  Swell the lyophilized powder in deionized water (use of some buffers can result in non-recoverable loss of volume) at 200 mL/g. Typically 90 to 95% of swelling occurs within 30 minutes at room temperature, but it may require overnight at 2 to 8 °C for 100% swelling to occur. After swelling, the agarose beads must be washed thoroughly with 10 volumes of water or equilibration buffer (such as PBS) to remove the lactose present in the lyophilized product.

  3. How is Product G4510, Glutathione-Agarose, resin conjugated to the glutathione, and what is the binding capacity of the resin?

    This product consists of glutathione attached through the sulfur to epoxy activated 4% cross-linked beaded agarose, resulting in a 12 atom spacer. Binding capacity: approx. 5 to 10 mg glutathione S-transferase per mL resin.

  4. When using Product G4510, Glutathione-Agarose, how do I store the swollen resin?

    After the resin has been swollen, it can be stored in 2 M NaCl at 2 to 8 °C. For long-term storage, a bacteriostatic agent such as 1 mM sodium azide or 0.02% thimerosal should be included. Alternatively, the resin can be relyophilized after addition of lactose.

  5. How do I regenerate Product G4510, Glutathione-Agarose, resin?

    Regeneration method:1. Wash with approximately 5 resin volumes of 0.1 M borate buffer, pH 8.5 containing 0.5 M NaCl (Cleansing Buffer 1).2. Wash with at least 5 column volumes of deionized water.3. Wash with at least 5 column volumes of 0.1 M acetate buffer, pH 4.5, containing 0.5 M NaCl (Cleansing Buffer 2).4. Wash with approximately 5 resin volumes of deionized water. For long-term storage, store in 2 M NaCl containing a bacteriostatic agent.5. Equilibrate with equilibration buffer before use.

  6. What reagents are compatible with Product G4510, Glutathione-Agarose, resin?

    Tris or phosphate buffers (pH 6.5 to 9.5) are typical lysis buffers compatible with glutathione affinity chromatography. Salt concentrations of up to 1 Molar do not interfere with binding. Protease inhibitors such as EDTA (Product No. E7889) or PMSF (Product No. P7626) are often included in the lysis buffer. Serine protease inhibitors included in the lysis buffer will not interfere with subsequent thrombin or factor Xa treatment as these inhibitors are removed before the proteolysis step. The binding of GST to glutathione-agarose is unaffected by 1% Triton X-100, 1% Tween-20, 1% CTAB, 10 mM DTT or 0.03% SDS.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Wenrong Tan et al.
PLoS genetics, 14(4), e1007336-e1007336 (2018-04-17)
Drought is a major threat to plant growth and crop productivity. The phytohormone abscisic acid (ABA) plays a critical role in plant response to drought stress. Although ABA signaling-mediated drought tolerance has been widely investigated in Arabidopsis thaliana, the feedback
Xiu Feng Hu et al.
The Journal of clinical investigation, 119(2), 362-375 (2009-01-17)
Provirus integration site for Moloney murine leukemia virus (PIM1) is a proto-oncogene that encodes a serine/threonine kinase with multiple cellular functions. Overexpression of PIM-1 plays a critical role in progression of prostatic and hematopoietic malignancies. Here we describe the generation
Dustin L Johnson et al.
Journal of bacteriology, 190(8), 2972-2980 (2008-02-19)
Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intracellular
Samie R Jaffrey et al.
Proceedings of the National Academy of Sciences of the United States of America, 99(5), 3199-3204 (2002-02-28)
The specificity of the reactions of nitric oxide (NO) with its neuronal targets is determined in part by the precise localizations of neuronal NO synthase (nNOS) within the cell. The targeting of nNOS is mediated by adapter proteins that interact
Hui Li et al.
Nature communications, 9(1), 2573-2573 (2018-07-04)
The dynamic maintenance of polar domains in the plasma membrane (PM) is critical for many fundamental processes, e.g., polar cell growth and growth guidance but remains poorly characterized. Rapid tip growth of Arabidopsis pollen tubes requires dynamic distribution of active
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