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If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

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主要文件

安全信息

GE17-0756-01

Glutathione Sepharose 4B

Cytiva 17-0756-01, pack of 10 mL

别名:

affinity chromatography resin, GST-tagged proteins purification,

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选择尺寸

20 μG
¥3,096.05
50 μG
¥5,521.21

¥3,096.05


预计发货时间2025年7月18日详情



选择尺寸

变更视图
20 μG
¥3,096.05
50 μG
¥5,521.21

About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

¥3,096.05


预计发货时间2025年7月18日详情


ligand

glutathione

包装

pack of 10 mL

制造商/商品名称

Cytiva 17-0756-01

基质

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

4-13

工作范围

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)

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EMU072451EMU085071EMU069331
esiRNA cDNA target sequence

TCATTCTCTTACAACCACCTTCCATATACCTTCAAGTCAACATTACCAAGAAGAGCATTCACCATCATTGTCAGGATCAGATTCTCTTCTTCAAATAACCACTCCTGTGGTGGCTTCAACTGTAGGTAACCCAAATCAGCATTCAGCAACAATGTCAACACCAGCTATTCATGTCTCAACTTATCACACGGCCCCTACTGAAGTGTCTGCTGCGTTTGAAGAACAGCCTGTCTCACCACACATTGGAGGAATGCCGTCACCAATTCAACACGATTTTCCTGCACTAGTGATGATACTCATAATTTTGGGCGTGATGGCAGGGATTATCGGAACTATCCTTCTTATCTCTTACTGTATCAGCCGAATGACAAAGAAAAGTTCAGTTGACATCCAATCTCCTGAGGGTGGTGACAACAGTGTGCCTTTGAGTTCTATTGAGCAGACTCCTAATGAAGAGTCCTCCAATGTTTGAAAGACCTCAAGGAAAACAAGACTTCAATCAATGGGAAA

esiRNA cDNA target sequence

GGGCAGGTTGAGTTCATTGTAAATAATTGTGTAAATCACTGTTCTAGTAATTTAAAAATAACATTAATTTTTCTACAAAATGCGAGATTTATAAATGTTTGAAATTGTACCTATTGATAATTAATGGTAAGTTTACTTAAAATAAATGTATCCCTTATTTCTTATCTGCATTTCTCATTTCCAGATGAAAGTTTAGTTATCAGAGATGTTTCAGCACACACAAAAGCCACTTTTCAGTACTTGTTCACATGCACTGACGAGCTAATTAGCAGCAGAAGTGGTTTCTGGAATGATGAACACCTTAATCATTCTGTTAGAACAGATTAGACAGTTCCTACCTCAGCATTTTCAGAAAACACACTTTCAAGGCCAAAAGCCATCATCATCAGTAGAGTCTCCCCTTGCTGAGACCTAATTATCTCCGGGGTTCCATC

esiRNA cDNA target sequence

TGACAGCGAAGGCAATGTAGTGAACAAACCCGACAGCCATGGTGGCAGCCTCGAATGGACAAAGATCTGTGAGAAGTCCTCTAAAGTGATCACCTTCATCGACTTGGCTGGCCACGAGAAGTACCTTAAGACCACTGTTTTTGGCATGACTGGCCACTTGCCTGACTTCTGCATGCTCATGGTGGGCAGCAATGCTGGCATTGTGGGGATGACCAAGGAGCATCTGGGCTTGGCATTGGCACTCAATGTACCTGTGTTTGTGGTTGTCACCAAGATTGACATGTGTCCTGCCAACATCCTTCAAGAAACCCTGAAGCTGCTACAGCGCCTGCTGAAGTCACCAGGCTGCCGGAAGATCCCTGTCCTGGTGCAGAGCAAAGATGATGTGATTGTCACAGCCTCCAACTTCAGCTCTGAGAGGATGTGCCCGATATTCCAGATCTCCAATGTCACAGGTGAGAACCTTGACCTGCTCAAGATGTTCCTCAACCTCCTGTCC

esiRNA cDNA target sequence

CACCAGTTTCGGACCAGTTTCAGACCACGATATGGAGTATACAATAGATGTGTTTTTCCGTCAAAGTTGGAAGGATGAAAGATTAAAATTCAAAGGACCCATGACAGTGCTCCGGCTAAACAACCTTATGGCCAGTAAAATCTGGACTCCAGATACATTTTTCCACAATGGAAAGAAGTCTGTGGCCCACAACATGACCATGCCTAATAAGCTCCTGCGTATCACAGAGGATGGCACTCTGCTGTACACCATGAGGTTGACCGTGAGAGCTGAATGCCCAATGCACTTGGAAGACTTCCCTATGGATGCCCATGCCTGCCCACTAAAATTCGGAAGCTATGCTTATACAAGAGCAGAAGTTGTCTATGAGTGGACCAGAGAGCCAGCCCGTTCAGTGGTTGTAGCAGAAGATGGGTCACGTTT

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

Ensembl | mouse accession no.

ENSMUSG00000051839

Ensembl | mouse accession no.

ENSMUSG00000025059

Ensembl | mouse accession no.

ENSMUSG00000042535

Ensembl | mouse accession no.

ENSMUSG00000010803

Gene Information

mouse ... GYPA(14934), Gypa(14934)

Gene Information

mouse ... GYK(14933), Gyk(14933)

Gene Information

mouse ... GTPBP1(14904), Gtpbp1(14904)

Gene Information

mouse ... GABRA1(14394), Gabra1(14394)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

一般描述

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

特点和优势

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids

法规信息

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    Lot/Batch Number

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    Xin Zhou et al.
    Nature communications, 11(1), 2674-2674 (2020-05-31)
    Increasing evidence indicates that guidance molecules used during development for cellular and axonal navigation also play roles in synapse maturation and homeostasis. In C. elegans the netrin receptor UNC-40/DCC controls the growth of dendritic-like muscle cell extensions towards motoneurons and
    Amandine Cartier-Michaud et al.
    PLoS genetics, 13(6), e1006803-e1006803 (2017-06-16)
    Spermatogenesis is a dynamic process that is regulated by adhesive interactions between germ and Sertoli cells. Germ cells express the Junctional Adhesion Molecule-C (JAM-C, encoded by Jam3), which localizes to germ/Sertoli cell contacts. JAM-C is involved in germ cell polarity
    Gerard H Pieper et al.
    Developmental cell, 53(1), 27-41 (2020-02-29)
    Eukaryotic genomes are organized within the nucleus through interactions with inner nuclear membrane (INM) proteins. How chromatin tethering to the INM is controlled in interphase and how this process contributes to subsequent mitotic nuclear envelope (NE) remodeling remains unclear. We have
    Ye Guan et al.
    eLife, 7 (2018-04-06)
    Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell
    Amandine Draime et al.
    Biochimica et biophysica acta, 1861(5), 534-542 (2018-02-23)
    Understanding how the activity of transcription factors like HOX proteins is regulated remains a widely open question. In a recent screen for proteins interacting with HOXA1, we identified a PRDM protein family member, PRDM14, which is known to be transiently

    商品

    This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.

    This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

    This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

    GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

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    实验方案

    This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

    This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

    GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

    This page how to remove GST tags by enzymatic cleavage using Cytiva products.

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