质量水平
形式
(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
分析物化学类别
proteins (GST)
包装
set of 3 pre-packed columns (2.5 ml each)
技术
immunoprecipitation (IP): suitable
protein purification: suitable
基质
cross-linked 4% beaded agarose
储存温度
2-8°C
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一般描述
这些柱中的树脂由通过硫连接至环氧活化的4%交联琼脂糖珠上的谷胱甘肽组成,形成12个原子间隔基。
应用
利用谷胱甘肽-琼脂糖的亲和层析技术可以快速、温和、非变性和高度选择性地纯化谷胱甘肽结合酶,如谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶和乙二醛酶I。
该产品用于新型色胺和四氢-β-咔啉基CDK4选择性抑制剂的设计、合成和生物学评价。它还可用于研究Fascaplysin激发的二吲哚作为CDK4/细胞周期素D1的选择性抑制剂。
该产品用于新型色胺和四氢-β-咔啉基CDK4选择性抑制剂的设计、合成和生物学评价。它还可用于研究Fascaplysin激发的二吲哚作为CDK4/细胞周期素D1的选择性抑制剂。
利用谷胱甘肽-琼脂糖亲和层析技术,可以快速、温和、非变性和高度选择性地纯化含有谷胱甘肽结合序列的蛋白质,如谷胱甘肽S-转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I。
联系
产品悬浮液编号G 4510
外形
0.5 M NaCl + 20%乙醇溶液的混悬液(1:1)
WGK
WGK 1
闪点(°F)
180.1 °F
闪点(°C)
82.3 °C
法规信息
新产品
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We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
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The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
相关内容
利用亲和力或GST沉降、串联亲和纯化(TAP)和免疫共沉淀法研究体外蛋白-蛋白相互作用所需的pull-down检测方法、试剂和实验方案。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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