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Merck
CN

G0924

Millipore

谷胱甘肽高容量磁性琼脂糖珠

(1:1 suspension in a 30% ethanol solution)

别名:

Magnetic agarose beads

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About This Item

MDL编号:
UNSPSC代码:
41106500
NACRES:
NA.56

偶联物

magnetic beads

质量水平

形式

(1:1 suspension in a 30% ethanol solution)

分析物化学类别

proteins (GST)

技术

immunoprecipitation (IP): suitable
protein purification: suitable

基质

4% beaded magnetic agarose

基质隔离区

12 atoms

容量

≥15 μmol/mL, agarose binding capacity

运输

wet ice

储存温度

2-8°C

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一般描述

谷胱甘肽磁性琼脂糖珠由顺磁性的4%珠状琼脂糖组成。谷胱甘肽通过硫共价连接到环氧活化琼脂糖上,形成12个原子(10个碳)的间隔基。

谷胱甘肽磁性琼脂糖珠可用于含有谷胱甘肽结合序列的蛋白质的亲和捕获、分子下拉或免疫沉淀反应(IP),如天然谷胱甘肽硫转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I,或来自细胞裂解物或其它生化溶液的GST标记重组融合蛋白,同时显示其它蛋白质的低非特异性结合。用磁铁分离结合到亲和树脂上的重组GST标记的蛋白质。磁性允许非常快速的加工,并有助于操作,例如重复洗涤和回收所需的蛋白质。这导致了更快的回收率、实验再现性和相关蛋白质的更精确定量。

磁珠基质是4%珠状琼脂糖,平均直径为50 μ;直径范围为20-75 μ。顺磁性铁浸渍在磁珠中。

应用

可用于含有谷胱甘肽结合序列的蛋白质的亲和捕获、分子下拉或免疫沉淀反应(IP),例如来自细胞裂解物和其他生物样本的重组GST标记的融合蛋白或天然谷胱甘肽转移酶、谷胱甘肽过氧化物酶和乙二醛酶I。可用于单管纯化或96孔多孔板高通量纯化。磁珠的磁性有助于操作,如重复洗涤和蛋白质回收。这导致了更高的实验再现性和相关蛋白质的更精确定量。

外形

30%乙醇溶液的混悬液(1:1)

象形图

Flame

警示用语:

Danger

危险声明

危险分类

Flam. Liq. 2

WGK

WGK 2

法规信息

危险化学品
动植物源性产品

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Xueran Chen et al.
Theranostics, 10(3), 998-1015 (2020-01-16)
Rationale: Glioblastoma multiforme (GBM) almost invariably gain invasive phenotype with limited therapeutic strategy and ill-defined mechanism. By studying the aberrant expression landscape of gliomas, we find significant up-regulation of p-MAPK level in GBM and a potent independent prognostic marker for
Yong-Wei Nie et al.
Biochemical and biophysical research communications, 473(1), 249-254 (2016-03-24)
Sorting motifs are involved in the transport of diverse proteins. In the present study, we identified a hydrophobic peptide (WRPWRNFWWSIRVPWRRN) that was able to target enhanced green fluorescent protein- or DsRed2-enriched vesicular-like sub-compartments of the endoplasmic reticulum (ER). Analysis of
Reinier F Prosée et al.
PLoS biology, 19(7), e3000968-e3000968 (2021-07-07)
Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes, CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In
Yun Wei et al.
Journal of materials chemistry. B, 1(15), 2066-2071 (2013-04-21)
The imidazolium cation was chosen as protein selective affinity group and a kind of ionic liquid magnetic microspheres was developed by immobilizing imidazolium cations onto the surface of silica-coated magnetic microspheres to form imidazolium ionic liquid modified magnetic microspheres (Fe3O4@SiO2@IL).
Ian Holt et al.
BMC molecular and cell biology, 21(1), 88-88 (2020-12-03)
Popeye domain-containing proteins 1 and 2 (POPDC1 and POPDC2) are transmembrane proteins involved in cyclic AMP-mediated signalling processes and are required for normal cardiac pacemaking and conduction. In order to identify novel protein interaction partners, POPDC1 and 2 proteins were

相关内容

利用亲和力或GST沉降、串联亲和纯化(TAP)和免疫共沉淀法研究体外蛋白-蛋白相互作用所需的pull-down检测方法、试剂和实验方案。

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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