偶联物
magnetic beads
质量水平
形式
(1:1 suspension in a 30% ethanol solution)
分析物化学类别
proteins (GST)
技术
immunoprecipitation (IP): suitable
protein purification: suitable
基质
4% beaded magnetic agarose
基质隔离区
12 atoms
容量
≥15 μmol/mL, agarose binding capacity
运输
wet ice
储存温度
2-8°C
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一般描述
谷胱甘肽磁性琼脂糖珠由顺磁性的4%珠状琼脂糖组成。谷胱甘肽通过硫共价连接到环氧活化琼脂糖上,形成12个原子(10个碳)的间隔基。
谷胱甘肽磁性琼脂糖珠可用于含有谷胱甘肽结合序列的蛋白质的亲和捕获、分子下拉或免疫沉淀反应(IP),如天然谷胱甘肽硫转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I,或来自细胞裂解物或其它生化溶液的GST标记重组融合蛋白,同时显示其它蛋白质的低非特异性结合。用磁铁分离结合到亲和树脂上的重组GST标记的蛋白质。磁性允许非常快速的加工,并有助于操作,例如重复洗涤和回收所需的蛋白质。这导致了更快的回收率、实验再现性和相关蛋白质的更精确定量。
磁珠基质是4%珠状琼脂糖,平均直径为50 μ;直径范围为20-75 μ。顺磁性铁浸渍在磁珠中。
谷胱甘肽磁性琼脂糖珠可用于含有谷胱甘肽结合序列的蛋白质的亲和捕获、分子下拉或免疫沉淀反应(IP),如天然谷胱甘肽硫转移酶(GST)、谷胱甘肽过氧化物酶和乙二醛酶I,或来自细胞裂解物或其它生化溶液的GST标记重组融合蛋白,同时显示其它蛋白质的低非特异性结合。用磁铁分离结合到亲和树脂上的重组GST标记的蛋白质。磁性允许非常快速的加工,并有助于操作,例如重复洗涤和回收所需的蛋白质。这导致了更快的回收率、实验再现性和相关蛋白质的更精确定量。
磁珠基质是4%珠状琼脂糖,平均直径为50 μ;直径范围为20-75 μ。顺磁性铁浸渍在磁珠中。
应用
可用于含有谷胱甘肽结合序列的蛋白质的亲和捕获、分子下拉或免疫沉淀反应(IP),例如来自细胞裂解物和其他生物样本的重组GST标记的融合蛋白或天然谷胱甘肽转移酶、谷胱甘肽过氧化物酶和乙二醛酶I。可用于单管纯化或96孔多孔板高通量纯化。磁珠的磁性有助于操作,如重复洗涤和蛋白质回收。这导致了更高的实验再现性和相关蛋白质的更精确定量。
外形
30%乙醇溶液的混悬液(1:1)
警示用语:
Danger
危险声明
危险分类
Flam. Liq. 2
WGK
WGK 2
法规信息
危险化学品
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相关内容
利用亲和力或GST沉降、串联亲和纯化(TAP)和免疫共沉淀法研究体外蛋白-蛋白相互作用所需的pull-down检测方法、试剂和实验方案。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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