生物来源
algae (marine)
质量水平
形式
suspension
技术
affinity chromatography: suitable
珠子直径
45-165 μm
孔径
30,000-50,000 Da fractionation range (Dextrans)
60,000-20,000,000 Da fractionation range (Globular proteins)
储存温度
2-8°C
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
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一般描述
琼脂糖凝胶™ 4B,一种琼脂糖珠,是Galβ1,4[3,6]-脱氢-L-半乳糖的聚合物。琼脂糖凝胶有助于分离较大的分子,并且没有带电荷的糖类。其含有3,6-脱水-L-半乳糖,可抵抗生物降解。氢键可稳定琼脂糖凝胶的结构。琼脂糖凝胶通过交联基质上的各种配体而广泛用于亲和层析。
应用
琼脂糖凝胶™ 4B已用于:
- 结合蝎毒以用于亲和层析
- 在免疫沉淀之前清除蛋白质提取物
- 凝胶纯化和磷脂囊泡的脱盐
- 免疫球蛋白G(IgG)的吸附
法律信息
Sepharose is a trademark of Cytiva
警示用语:
Warning
危险声明
危险分类
Flam. Liq. 3
WGK
WGK 1
闪点(°F)
100.4 - 109.4 °F
闪点(°C)
38 - 43 °C
法规信息
危险化学品
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Nature Protocols, 10(6), 927-927 (2015)
Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice.
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Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
PloS one, 14(8), e0220866-e0220866 (2019-08-08)
Dipeptidyl amino-peptidase 3 (DPP3) is an aminopeptidase involved in peptide degradation, including hormone peptides as angiotensin II and enkephalins. DPP3 plasma activity increases in septic patients and correlates with mortality risk. However, the exact physiological role of DPP3 remains unclear
Journal of virology, 81(11), 5579-5593 (2007-03-16)
The human immunodeficiency virus type 1 exterior gp120 envelope glycoprotein is highly flexible, and this flexibility may contribute to the inability of monomeric gp120 immunogens to elicit broadly neutralizing antibodies. We previously showed that an S375W modification of a critical
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