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Merck
CN

SAB4200692

Sigma-Aldrich

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal

clone GST-2, purified from hybridoma cell culture

别名:

GST, Glutathione-S-Transferase

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

GST-2, monoclonal

表单

buffered aqueous solution

浓度

~1 mg/mL

技术

ELISA: suitable
dot blot: suitable
immunoblotting: 0.5 μg/mL
immunofluorescence: suitable
immunoprecipitation (IP): suitable

同位素/亚型

IgG2b

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

一般描述

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma GST-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant GST fusion protein. Glutathione-S-Transferase (GST) is a 26 kDa protein found in eukaryotic cells. It belongs to the phase II detoxification enzymes family.

免疫原

purified recombinant GST fusion protein

应用

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • dot blotting
  • autoradiography
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)

生化/生理作用

Glutathione-S-Transferase (GST) helps to catalyze the conjugation of electrophilic substrates to reduced form of glutathione (GSH). Genetic engineering uses glutathione-S-transferase (from parasitic helminth Schistosoma japonicum) to create the GST gene fusion system for production of GST-labeled recombinant proteins. GST-fusion proteins were successfully demonstrated in structure- function studies involving protein-protein and DNA protein interactions via GST pull down assays.

外形

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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分析证书(COA)

Lot/Batch Number

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访问文档库

Viral pathogens hitchhike with insect sperm for paternal transmission
Mao Q, et al.
Nature Communications, 10(955), 1-10 (2019)
Manhui Xia et al.
OncoTargets and therapy, 12, 1575-1582 (2019-03-19)
Senescence has been recognized as a mechanism for the suppression of tumorigenesis. However, how the senescence is regulated is not fully understood. The present study aims to elucidate MLL3-mediated regulation of senescence. MLL3 protein levels in esophageal squamous cell carcinoma
MLL3 promotes the senescence of esophageal squamous cell carcinoma
Xia M, et al.
OncoTargets and Therapy, 12, 1575-1575 (2019)
Naveen Malik et al.
The Plant journal : for cell and molecular biology, 101(6), 1411-1429 (2019-11-09)
Mediator, a multisubunit co-activator complex, regulates transcription in eukaryotes and is involved in diverse processes in Arabidopsis through its different subunits. Here, we have explored developmental aspects of one of the rice Mediator subunit gene OsMED14_1. We analyzed its expression
Qianzhuo Mao et al.
Nature communications, 10(1), 955-955 (2019-03-01)
Arthropod-borne viruses (arboviruses) can be maternally transmitted by female insects to their offspring, however, it is unknown whether male sperm can directly interact with the arbovirus and mediate its paternal transmission. Here we report that an important rice arbovirus is

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