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Merck
CN

CS0410

Sigma-Aldrich

谷胱甘肽S-转移酶(GST)活性检测试剂盒

sufficient for 500 multiwell tests

别名:

GST Enzyme Activity Kit

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About This Item

EC 号:
UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 500 multiwell tests

质量水平

运输

dry ice

储存温度

−20°C

基因信息

一般描述

谷胱甘肽S-转移酶 (GST) 检测试剂盒用于测定总GST的活性。适用于细胞和细菌裂解物、组织匀浆以及血浆
和红细胞裂解物样品。

应用

该谷胱甘肽S-转移酶检测试剂盒采用1-氯-2,4-二硝基苯(CDNB),适用于广泛的GST同工酶。基本原理是谷胱甘肽硫醇基团结合到CDNB底物上后,在340nm处的吸光度会增加。

生化/生理作用

谷胱甘肽S-转移酶 (GST)是在哺乳动物解除许多外源物毒素过程中发挥重要作用的一组酶。该酶可将谷胱甘肽的硫醇基团连接到亲电的外源物上,保护细胞免受毒物损害,进一步帮助细胞抵抗化合物诱变、致癌和毒性作用。GST活性广泛存在于植物、昆虫、酵母、细菌以及大多数哺乳动物组织中,尤其是在解毒过程中发挥关键作用的肝脏中。

仅试剂盒组分

产品编号
说明

  • Dulbecco's Phosphate Buffered Saline 100 mL

  • L-Glutathione Reduced 1 g

  • GST (Control) .1 mL

  • Sample Buffer 5 mL

  • Substrate (CDNB) 1.2 mL

警示用语:

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

危险化学品
常规特殊物品
含少量动物源组分生物产品

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T Lin et al.
Toxicology, 235(1-2), 1-10 (2007-04-10)
Glutathione (GSH) is one of the most important antioxidants in mammalian cells. It also plays an important role in chemical detoxification. Some evidence showed that polycyclic aromatic hydrocarbons, such as benzo[a]pyrene (B[a]P [50-32-8]), could increase GSH content as a defense
S Melino et al.
Amino acids, 41(1), 103-112 (2010-03-10)
Natural organosulfur compounds (OSCs) have been shown to have chemopreventive effects and to suppress the proliferation of tumor cells in vitro through the induction of apoptosis. The biochemical mechanisms underlying the antitumorigenic and anti-proliferative effects of garlic-derived OSCs are not
Daniel Scotcher et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(5), 556-568 (2017-03-09)
In vitro-in vivo extrapolation of drug metabolism data obtained in enriched preparations of subcellular fractions rely on robust estimates of physiologically relevant scaling factors for the prediction of clearance in vivo. The purpose of the current study was to measure
Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.
W H Habig et al.
The Journal of biological chemistry, 249(22), 7130-7139 (1974-11-25)
Shunying Yang et al.
BMC plant biology, 20(1), 143-143 (2020-04-09)
Overuse of nitrogen fertilizers is often a major practice to ensure sufficient nitrogen demand of high-yielding rice, leading to persistent NH4+ excess in the plant. However, this excessive portion of nitrogen nutrient does not correspond to further increase in grain

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