一般描述
谷胱甘肽S-转移酶 (GST) 检测试剂盒用于测定总GST的活性。适用于细胞和细菌裂解物、组织匀浆以及血浆
和红细胞裂解物样品。
和红细胞裂解物样品。
应用
该谷胱甘肽S-转移酶检测试剂盒采用1-氯-2,4-二硝基苯(CDNB),适用于广泛的GST同工酶。基本原理是谷胱甘肽硫醇基团结合到CDNB底物上后,在340nm处的吸光度会增加。
生化/生理作用
谷胱甘肽S-转移酶 (GST)是在哺乳动物解除许多外源物毒素过程中发挥重要作用的一组酶。该酶可将谷胱甘肽的硫醇基团连接到亲电的外源物上,保护细胞免受毒物损害,进一步帮助细胞抵抗化合物诱变、致癌和毒性作用。GST活性广泛存在于植物、昆虫、酵母、细菌以及大多数哺乳动物组织中,尤其是在解毒过程中发挥关键作用的肝脏中。
仅试剂盒组分
产品编号
说明
- Dulbecco's Phosphate Buffered Saline 100 mL
- L-Glutathione Reduced 1 g
- GST (Control) .1 mL
- Sample Buffer 5 mL
- Substrate (CDNB) 1.2 mL
警示用语:
Danger
危险分类
Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
危险化学品
常规特殊物品
含少量动物源组分生物产品
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