AC113
Anti-Neomycin Phosphotransferase II Antibody | AC113
from rabbit, purified by affinity chromatography
别名:
Aminoglycoside 3′-phosphotransferase, APH(3′)-II, APH(3′)II, Kanamycin kinase, type II, Neomycin-kanamycin phosphotransferase type II
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所有图片(1)
About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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一般描述
Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
特异性
This antibody reacts to lysates from cells transfected or transformed with a plasmid containing a kanamycin/neomycin resistance gene, nptII (Aminoglycoside 3′-phosphotransferase).
免疫原
Recombinant protein corresponding to full length neomycin phosphotransferase (NPT) II.
应用
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
质量
Evaluated by Western Blot in untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neomycin phosphotransferase II in 10 µg of neomycin treated HEK293 cells transfected with pUSEamp(-).
目标描述
~29 kDa observed.
外形
Affinity purified
Hek 293 +/- transfection with pUSEamp(-)and neomycin treatment.
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).
Untreated and neomycin treated HEK293 cells transfected with pUSEamp(-).
法律信息
Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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