一般描述
SYPRO Orange与SYPRO Red相似，但它颜色更亮一些，背景荧光略高。 对于使用激光激发凝胶扫描仪的用户，推荐使用一种基于氩离子激光的SYPRO Orange仪器。 该染料可由紫外线或宽带照明光有效激发。它不适用于印迹或IEF凝胶中蛋白的染色，并在2维凝胶染色中显示出较低的灵敏度。
SYPRO Orange为：
SYPRO Orange为：
- 高灵敏度。 该染色剂可检测每minigel条带中1-2ng的蛋白质，比考马斯亮蓝或银染色更灵敏。
- 快速。 在不到1小时内完成染色。
- 简单。 电泳后，对凝胶进行染色、冲洗并拍照；不需要固定或脱色步骤
- 与标准实验室设备兼容。 染色蛋白可以用标准的300纳米紫外透照仪或激光扫描仪来观察。
- 物美价廉。 SYPRO Orange染色法较银染法便宜，并且所需时间短。
- 不同蛋白间变化小。 染料与蛋白质周围的SDS包被层相互作用，使不同类型的蛋白质之间的染色比考马斯或银染色更一致。
- 对蛋白的选择性。 SYPRO Orange检测的蛋白质小至6.5 kDa，不染色核酸或脂多糖。 它染色糖基化蛋白。
- 较宽的检测线性范围。 染色条带的荧光强度与超过3个数量级的蛋白量呈线性相关(比考马斯染色或银染色范围更广)。
应用
SYPRO Orange蛋白凝胶染色技术已应用于蛋白质的热变性和热转移实验。它也被用于微分扫描荧光法。
注意
SYPRO储液应在室温、4°C或20°C下干燥保存并避光。如果储存得当，这些储液可以稳定使用6个月到1年。使用缓冲液或醋酸溶液稀释后的染色试剂可以储存在非常干净、无去污剂的玻璃瓶或塑料瓶中，在4℃下避光保存至少3个月。
法律信息
SYPRO is a registered trademark of Life Technologies
相关产品
WGK
WGK 3
Influence of GTP on system specific chaperone - Twin arginine signal peptide interaction.
Cherak SJ and Turner RJ
Biochemical and Biophysical Research Communications, 465, 753-757 (2015)
Ruth Cohen-Khait et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(52), 14982-14987 (2016-12-14)
Protein-protein interactions occur via well-defined interfaces on the protein surface. Whereas the location of homologous interfaces is conserved, their composition varies, suggesting that multiple solutions may support high-affinity binding. In this study, we examined the plasticity of the interface of
Mohammad M Rahman et al.
Journal of bacteriology, 201(20) (2019-07-31)
The gastric pathogen Helicobacter pylori has limited ability to use carbohydrates as a carbon source, relying instead on exogenous amino acids and peptides. Uptake of certain peptides by H. pylori requires an ATP binding cassette (ABC) transporter annotated dipeptide permease
Xiahezi Kuai et al.
Frontiers in plant science, 8, 1715-1715 (2017-10-20)
Each year, crop yield is lost to weeds competing for resources, insect herbivory and diseases caused by pathogens. To thwart these insults and preserve yield security and a high quality of traits, conventional agriculture makes use of improved cultivars combined
Brijesh B Khadgi et al.
Nucleic acids research, 47(16), 8708-8719 (2019-08-09)
Long Interspersed Elements (LINEs), also known as non-LTR retrotransposons, encode a multifunctional protein that reverse transcribes its mRNA into DNA at the site of insertion by target primed reverse transcription. The second half of the integration reaction remains very poorly
商品
为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
实验方案
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门