一般描述
SYPRO Ruby 蛋白凝胶染色剂是一种即用型、超灵敏的发光染色剂，用于检测聚丙烯酰胺凝胶电泳 (PAGE) 分离的蛋白。该染色剂专为 2-D PAGE 设计，已被证明是 1-D PAGE 和等电聚焦 (IEF) 凝胶的极佳选择。SYPRO 红宝石蛋白凝胶染色的灵敏度与许多银染技术相当。但是，与银染不同的是，SYPRO Ruby 凝胶染色：
- 使用简单的染色方案，无过度染色的可能性
- 提供超过三个数量级的线性定量范围
- 显示较少的蛋白质间变异性
- 染色糖蛋白、脂蛋白、钙结合蛋白、纤维状蛋白和其他难染色蛋白
- 不会染色外源性核酸
- 不会干扰基于 Edman 测序或质谱的蛋白质后续分析
应用
SYPRO 红宝石蛋白凝胶染色剂用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 后的蛋白染色。
注意
避光保存。
法律信息
SYPRO is a registered trademark of Life Technologies
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Chronic hypoxia alters mitochondrial composition in human macrophages.
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Antibody microarrays are a multiplexing technique for the analyses of hundreds of different analytes in parallel from small sample volumes of few microlitres only. With sensitivities in the picomolar to femtomolar range, they are gaining importance in proteomic analyses. These
商品
为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
实验方案
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
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