跳转至内容
Merck
CN
所有图片(1)

文件

G1041

Sigma-Aldrich

EZBlue ™ 凝胶染色试剂

别名:

protein gel stain, protein stain

登录查看公司和协议定价
所有图片(1)

About This Item

UNSPSC代码:
41105300
NACRES:
NA.32

技术

protein staining: suitable

质量水平

200

储存温度

2-8°C

相关类别

一般描述

EZBlue ™ 考马斯亮蓝 G-250 胶体蛋白染色方便、灵敏、安全，在显著减少染色时间的同时，改善了蛋白电泳结果。EZBlue包装方便， 不需要忙乱的称重或添加甲醇或酸。作为胶体染色剂，它只与蛋白质反应，而不与凝胶本身反应。背景染色减少，因此蛋白条带几乎可以立即显示出来。无需脱色步骤，但水洗可能会强化条带并澄清背景。最令人印象深刻的是，EZBlue 极为灵敏，可检测低至 5 ng 的蛋白质。

应用

EZBlue 凝胶染色试剂已被用作十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 中的染色试剂。

特点和优势

  • 预混溶液 省去了制备染色剂所需的时间和精力
  • 灵敏度的提高 确保低丰度蛋白（低至 5 ng）可被检测到
  • 快速反应 显著减少了染色和冲洗所需的时间
  • 无溶剂废物 ，因此可以通过消除有害物质处置步骤来节省时间和金钱

其他说明

即用型亮蓝 G-250 基蛋白染色剂，用于聚丙烯酰胺凝胶和 PVDF 膜上的“一步”超灵敏检测。

法律信息

EZBlue is a trademark of Sigma-Aldrich Co. LLC
SIGMA-ALDRICH is a registered trademark of Merck KGaA, Darmstadt, Germany

相关产品

产品编号
说明
价格

象形图

Health hazardCorrosion
GHS08,GHS05

警示用语：

Warning

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

靶器官

Eyes,Central nervous system

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

AMPA receptor antibodies in limbic encephalitis alter synaptic receptor location.
Lai M, et al.
Annals of Neurology, 65, 424-434 (2009)
Praveen Maurye et al.
Electrophoresis, 38(16), 2060-2068 (2017-04-27)
PAGE is the most widely used technique for the separation and biochemical analysis of biomolecules. The ever growing field of proteomics and genomics necessitates the analysis of many proteins and nucleic acid samples to understand further about the structure and
Rakhi Panda et al.
Journal of agricultural and food chemistry, 63(43), 9629-9639 (2015-10-09)
Many soybean protein products are processed by enzymatic hydrolysis to attain desirable functional food properties or in some cases to reduce allergenicity. However, few studies have investigated the effects of enzymatic hydrolysis on the allergenicity of soybean products. In this
Nathan L Marsteller et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 141, 111398-111398 (2020-05-22)
Currently no validated animal model is predictive of human responses in ranking purified dietary proteins in the prevalence or potency of food allergy in humans. Since the gastrointestinal microbiota is thought to influence oral tolerance, we hypothesize that a germ-free
Antibodies to the GABA(B) receptor in limbic encephalitis with seizures: case series and characterisation of the antigen.
Lancaster E, et al.
Lancet Neurology, 9, 67-76 (2010)
查看所有相关文献

商品

Cell Lysate Preparation

Cell lysis challenges and protein extraction variables for Western blot sensitivity and reproducibility.

Western Blot Analysis of Immunoprecipitation (IP-Western)

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

蛋白电泳染色剂选择指南

为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。

查看所有结果

实验方案

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌制PAGE和SDS-PAGE凝胶

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门