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Merck
CN

PROTSIL1

ProteoSilver™ 银染试剂盒

别名:

银染试剂盒

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NACRES:
NA.32
UNSPSC Code:
41105322

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usage

 kit sufficient for 25 mini-gels (10 × 10 cm)

technique(s)

protein staining: suitable

Quality Level

200

相关类别

General description

ProteoSilver具有便于使用的包装，稳定的性能，极高的灵敏度，适合任何蛋白研究科学家使用。该试剂盒含有制备好的银染试剂，并配有如何获得最佳结果的详细说明。ProteoSilver的检出限为0.1 ng/mm2的蛋白质(BSA)，本底极低，可对低丰度蛋白进行出色的检测。

Application

ProteoSilver™ 银染试剂盒已被用于凝胶染色以对蛋白进行显色。

Features and Benefits

  • 经预混和预称重 的溶液可减少对每一成分单独进行采购及制备所花费的时间和成本。
  • 经优化的实验方案 有助于确定获得最佳结果所需的条件。
  • 其高度的灵敏度及极低的本底 可确保对低丰度蛋白的检测并从其他蛋白中分辨出来。
  • 兼容于MALDI 感兴趣蛋白点可通过质谱进行进一步的定性分析。
  • 室温稳定性使得其保存容易而且方便。

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

存储类别

3 - Flammable liquids

flash_point_f

132.8 °F - closed cup

flash_point_c

56 °C - closed cup

法规信息

危险化学品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000510452
0000510452
0000441870
0000441870
0000368055

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Jean-Paul Lasserre et al.
Electrophoresis, 27(16), 3306-3321 (2006-07-22)
Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of
Yong-Feng Han et al.
The Plant cell, 28(5), 1215-1229 (2016-04-27)
The mechanism by which MORPHEUS' MOLECULE1 (MOM1) contributes to transcriptional gene silencing has remained elusive since the gene was first identified and characterized. Here, we report that two Arabidopsis thaliana PIAS (PROTEIN INHIBITOR OF ACTIVATED STAT)-type SUMO E3 ligase-like proteins
Yufan Zhang et al.
Materials today. Bio, 15, 100317-100317 (2022-06-28)
Polymer dots (Pdots) have been applied to imaging lymph nodes (LNs) and lymphatic vessels (LVs) in living mice and rats. However, the mechanism of absorption, distribution, metabolism, and excretion of Pdots in LNs and LVs is still unclear. Therefore, the
Andrea Miccoli et al.
International journal of molecular sciences, 22(7) (2021-04-04)
In jawed vertebrates, adaptive immune responses are enabled by T cells. Two lineages were characterized based on their T cell receptor (TcR) heterodimers, namely αβ or γδ peptide chains, which display an Ig domain-type sequence that is somatically rearranged. γδ
Zongyu Gao et al.
Nature communications, 13(1), 716-716 (2022-02-09)
Mitogen-activated protein kinase (MAPK) cascades play an important role in innate immunity against various pathogens in plants and animals. However, we know very little about the importance of MAPK cascades in plant defense against viral pathogens. Here, we used a
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商品

Bis Tris Polyacrylamide Gel Electrophoresis Technology

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

蛋白电泳染色剂选择指南

为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。

实验方案

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌制PAGE和SDS-PAGE凝胶

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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