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安全信息

F7524

Sigma-Aldrich

胎牛血清

non-USA origin, sterile-filtered, suitable for cell culture

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别名:
FBS, FCS, 血清
MDL编号:
MFCD00132239
UNSPSC代码:
12352207
eCl@ss:
32160409
NACRES:
NA.71

生物来源

bovine fetus

质量水平

400

无菌性

sterile-filtered

产品线

FBS Classic

组成

hemoglobin, ≤25 mg/dL

来源

non-USA origin

存货情况

not available in Canada, USA

技术

cell culture | mammalian: suitable

杂质

≤10 EU/mL endotoxin

运输

dry ice

储存温度

−20°C

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应用

胎牛血清已被用作细胞培养基中的补充剂，如Roswell Park Memorial Institute 培养基 (RPMI-1640) 和Dulbecco′s 改良Eagles 培养基(DMEM)，用于维持各种细胞系的生长。

生化/生理作用

胎牛血清(FBS) 是一种常用的细胞培养基补充剂，用于维持细胞、组织和器官的 体外培养。FBS仍然是一种常用的培养基补充剂，因为它在细胞培养中提供了广泛的功能。FBS提供营养、生长和附着因子，并通过难以在无血清培养基（SFM）系统中复制的机制保护细胞免受氧化损伤和细胞凋亡。它为培养的细胞提供多种未确定的生长促进和存活增强因子。它来源于凝结血，是真核细胞，尤其是哺乳动物细胞培养中最广泛使用的未确定补充剂。 FBS通常与在1950年代和1960年代开发的经典培养基一起使用。这些包括多种常用培养基，如Minimum Essential培养基（MEM）、Dulbecco′改良Eagle培养基（DME, DMEM）、Iscove′s改良Eagles培养基(IMDM)、M199以及Roswell Park Memorial Institute培养基RPMI-1640。

特点和优势

F7524是一个等同于Biochrom GmbH S0115产品的强大产品。

分析说明

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

法律信息

不在美国或加拿大销售。 有关贵国的销售情况和进口限制，请咨询您当地的供应商。

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WGK 3

闪点(°F)

Not applicable

闪点(°C)

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个人防护装备

Eyeshields, Gloves

法规信息

监管及禁止进口产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Is product F7524 heat inactivated?

    Not all Fetal Bovine Serum products are heat inactivated. Product F9665 is the heat-inactivated version of F7524.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. Is Product F7524, Fetal Bovine Serum, available in the US or Canada?

    No, Product No. F7524 is only available in Europe.

  5. Where is Product F7524, Fetal Bovine Serum, sourced from?

    Product is South American Origin and EU approved.  Some batches could be sourced from France.

  6. Is Product F7524, Fetal Bovine Serum, a USDA approved serum?

    This is a non-USDA approved serum. The serum is tested for BVD (bovine virus diarrhea), PI3 (parainfluenza type 3), and IBR (infectious bovine rhinotracheitis) only.

  7. How is Product F7524, Fetal Bovine Serum, sterilized?

    This product is sterilized by filtration through three (3) sterile filter membranes, each of which has a pore size of 0.1 microns.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. Does Sigma-Aldrich offer delipidated bovine serum?

    At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss

  11. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ruairi C Robertson et al.
Brain, behavior, and immunity, 59, 21-37 (2016-07-18)
Neurodevelopment is strongly influenced by maternal and early-postnatal diet. Omega-3 polyunsaturated fatty acids (n-3 PUFA) are vital structural and functional components of the developing brain. The gut microbiota is also influenced by n-3 PUFA status, however, little is known about
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
Maria Peiris-Pagès et al.
Aging, 11(14), 4801-4835 (2019-07-18)
Using proteomics analysis, we previously compared MCF7 breast cancer cells grown as 3D tumor spheres, with the same cell line grown as monolayers. Our results indicated that during 3D anchorage-independent growth, the cellular machinery associated with i) mitochondrial biogenesis and
Carlo E A Jochems et al.
Alternatives to laboratory animals : ATLA, 30(2), 219-227 (2002-04-25)
Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.
Elisabeth F P Peterse et al.
BMC cancer, 16, 475-475 (2016-07-16)
Chondrosarcoma is a malignant cartilage forming bone tumour for which no effective systemic treatment is available. Previous studies illustrate the need for a better understanding of the role of the IGF pathway in chondrosarcoma to determine if it can be
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