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Merck
CN
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文件

安全信息

12103C

Sigma-Aldrich

胎牛血清

USA origin, suitable for cell culture

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别名:
FBS, FCS, 血清
MDL编号:
MFCD00132239
UNSPSC代码:
12352207
NACRES:
NA.71

生物来源

bovine fetus

质量水平

500

产品线

FBS Premier

组成

hemoglobin, ≤20 mg/dL

来源

USA origin

技术

cell culture | mammalian: suitable

杂质

≤10 EU/mL endotoxin

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

FBS仍然是一种常用的培养基补充剂，因为它在细胞培养中提供了广泛的功能。FBS提供营养、生长和附着因子，并通过难以在无血清培养基（SFM）系统中复制的机制保护细胞免受氧化损伤和细胞凋亡。

应用

胎牛血清在不同类型的细胞培养物中用作补充剂，例如：
  • 在添加胎牛血清和其它成分的 Dulbecco ′改良 Eagle′培养基中PC12 细胞的生长。
  • HeLa 细胞是在含有胎牛血清和其它成分的罗斯威尔公园纪念研究所 (RPMI) 1640 培养基中供养。
  • BaF3 细胞培养物（细胞在补充胎牛血清和其它成分的 RPMI 1640 培养基中供养）。
  • 原代海马细胞培养物（细胞在补充有热灭活胎牛血清和其它成分的Eagle′最低必需培养基供养）。
  • HeLa、Cos1 和 Cos7 细胞在含有胎牛血清和其它成分的 Dulbecco 改良 Eagle 培养基 （DMEM） 中培养。
胎牛血清（FBS）由于其高营养成分，是用于补充细胞培养基的最常见的血清。尽管其在蛋白中含量相对较低，但FBS可有效促进和维持脊椎动物、哺乳动物和昆虫细胞的生长

该产品用于进一步的制造用途。本产品不用于人类或治疗用途。

分析说明

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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The serotypes of adeno-associated virus (AAV) have the potential to become important resources for clinical gene therapy. In an effort to compare the role of serotype-specific virion shells on vector transduction, we cloned each of the serotype capsid coding domains
Julie L Webb et al.
The Journal of biological chemistry, 278(27), 25009-25013 (2003-04-30)
Parkinson's disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra and the formation of aggregates (Lewy bodies) in neurons. alpha-Synuclein is the major protein in Lewy bodies and rare mutations in alpha-synuclein cause early-onset PD.
Tadepally Lakshmikanth et al.
Cell reports, 32(3), 107923-107923 (2020-07-23)
The human immune system varies extensively between individuals, but variation within individuals over time has not been well characterized. Systems-level analyses allow for simultaneous quantification of many interacting immune system components and the inference of global regulatory principles. Here, we
D B Bregman et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(21), 11586-11590 (1996-10-15)
Damage to actively transcribed DNA is preferentially repaired by the transcription-coupled repair (TCR) system. TCR requires RNA polymerase II (Pol II), but the mechanism by which repair enzymes preferentially recognize and repair DNA lesions on Pol II-transcribed genes is incompletely
R J Mark et al.
Journal of neurochemistry, 68(1), 255-264 (1997-01-01)
Peroxidation of membrane lipids results in release of the aldehyde 4-hydroxynonenal (HNE), which is known to conjugate to specific amino acids of proteins and may alter their function. Because accumulating data indicate that free radicals mediate injury and death of
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Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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