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一般描述
ProteoSilver™ Plus银染试剂盒具有方便的包装、极稳定的性能和高灵敏度，是所有蛋白质研究科学家的理想选择。 与ProteoSilver（PROTSIL1）相似，该试剂盒含有制备好的银染试剂，并配有如何获得最佳结果的详细说明。 ProteoSilver Plus的检出限为0.1 ng/mm2 的蛋白质（BSA），本底极低，可对低丰度蛋白进行出色的检测。
ProteoSilver Plus也可用于MALDI-MS检测，其不含会使赖氨酸残基发生交联的戊二醛，该成分会导致质谱结果失准。ProteoSilver Plus银染试剂盒另外含有两种可使切离蛋白点脱色的试剂，适于希望通过胰蛋白酶消化和MALDI-MS分析来进一步研究蛋白特性的研究人员。
ProteoSilver Plus也可用于MALDI-MS检测，其不含会使赖氨酸残基发生交联的戊二醛，该成分会导致质谱结果失准。ProteoSilver Plus银染试剂盒另外含有两种可使切离蛋白点脱色的试剂，适于希望通过胰蛋白酶消化和MALDI-MS分析来进一步研究蛋白特性的研究人员。
应用
ProteoSilver 银染试剂盒已可用于凝胶染色，以便观察显色的蛋白质。该试剂盒提供的脱色剂可用于使感兴趣的蛋白脱色，以便进行质谱分析。
特点和优势
- 经预混和预称重的溶液可减少对每一成分单独进行采购及制备所花费的时间和成本。
- 经优化的实验方案 有助于确定获得最佳结果所需的条件。
- 其高度的灵敏度及极低的本底可确保对低丰度蛋白的检测并从其他蛋白中分辨出来。
- 兼容于MALDI感兴趣蛋白点可通过质谱进行进一步的定性分析。
- 室温稳定性 使得其保存容易而且方便。
法律信息
ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Danger
危险分类
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3
靶器官
Eyes,Central nervous system, Respiratory system
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
149.0 °F - closed cup
闪点(°C)
65 °C - closed cup
法规信息
危险化学品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Slovénie Pyndiah et al.
Molecular & cellular proteomics : MCP, 6(2), 193-206 (2006-11-10)
The study of protein interactions constitutes an important domain to understand the physiology and pathogenesis of microorganisms. The two-dimensional blue native/SDS-PAGE was initially reported to analyze membrane protein complexes. In this study, both cytoplasmic and membrane complexes of a bacterium
Cédric Bernarde et al.
Molecular & cellular proteomics : MCP, 9(12), 2796-2826 (2010-07-09)
Helicobacter pylori infection plays a causal role in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma (LG-MALT) and duodenal ulcer (DU). Although many virulence factors have been associated with DU, many questions remain unanswered regarding the evolution of the
Peeping into human renal calcium oxalate stone matrix: characterization of novel proteins involved in the intricate mechanism of urolithiasis.
Aggarwal KP, et al.
PLoS ONE, 8, e69916-e69916 (2013)
Lina Fassi Fehri et al.
Antimicrobial agents and chemotherapy, 49(10), 4154-4165 (2005-09-29)
Antimicrobial peptides are widely distributed in nature, and in vertebrates, they play a key function in the innate immune defense system. It is generally agreed that these molecules may provide new antibiotics with therapeutic value. However, there are still many
Priyadarshini Pathak et al.
International braz j urol : official journal of the Brazilian Society of Urology, 36(5), 621-628 (2010-11-04)
Investigate the activity of high and low molecular weight biomolecules present in the matrix of human calcium oxalate (CaOx) stones not only on the initial mineral phase formation of calcium and phosphate (CaP) but also on its growth and demineralization
商品
为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
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