N6537
Anti-NPTII antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
别名:
Anti-Neomycin Phosphotransferase II
产品名称
Anti-NPTII antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 30 kDa
species reactivity
plant
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
western blot: 2-4 μg/mL using extract (cytosolic fraction) from the leaves of transgenic Nicotiana tabacum expressing E. coli NPTII
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Application
Anti-NPTII antibody has been used in immunoblotting.
Biochem/physiol Actions
The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.
The antibody recognizes bacterial NPTII expressed in transgenic tobacco plants.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from the E. coli transposon Tn5.
Immunogen
synthetic peptide corresponding to amino acids 133-150 located at the mid-region of E. coli NPTII, conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure
Yenofsky R L, et al.
Proceedings of the National Academy of Sciences of the USA, 87(9), 3435-3439 (1990)
Purification and characterization of microbially expressed neomycin phosphotransferase II (NPTII) protein and its equivalence to the plant expressed protein
Fuchs R L, et al.
Biotechnology, 11(12), 1537-1537 (1993)
相关内容
Datasheet
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