产品名称
组织蛋白酶D 人, recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 43.6 kDa
observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)
packaging
pkg of 10 μg
manufacturer/tradename
Sigma-Aldrich
storage condition
dry at room temperature
technique(s)
activity assay: suitable
impurities
<1 EU/μg endotoxin (LAL test)
suitability
suitable for molecular biology
UniProt accession no.
application(s)
life science and biopharma
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CTSD(1509)
Application
Cathepsin D has been used in the protease digestion of haemoglobin.
Biochem/physiol Actions
Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy. Elevated circulating CatD is observed in type 2 diabetes and can be considered an important biomarker for type 2 diabetes-dependent cardiac dysfunction. Increased CatD, is observed in non-alcoholic steatohepatitis.Cathepsin D is involved in various physiological processes like apoptosis, autophagy, and protein degradation. Cathepsin D plays an important role in the pathogenesis of Alzheimer’s disease, neuronal ceroid lipofuscinosis, and breast cancer.
General description
Research area: Cell signalling. Cathepsin D belongs to the peptidase A1 family, an estrogenic-induced lysosomal protease. Cathepsin D can be cleaved into the following 2 chains: N-terminal light chain and C-terminal heavy chain, which is expressed in the aorta extracellular space (at the protein level). ). It is found in most mammalian cells and is located in thelysosomes.
This gene is mapped to human chromosome 11p15.5.
Physical form
Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Increased Cathepsin D Correlates with Clinical Parameters in Newly Diagnosed Type 2 Diabetes
Liu L, et al.
Disease Markers, 2017(1), 23-28 (2017)
Cluster analysis of risk factor genetic polymorphisms in Alzheimer?s disease
Randall CN, et al.
Neurochemical Research, 34(1), 23-28 (2009)
Partial Characterization of Two Cathepsin D Family Aspartic Peptidases of Clonorchis sinensis
Jung-Mi Kang, et al.
The Korean Journal of Parasitology, 57(6), 671?680-671?680 (2019)
Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention
Walenbergh SMA, et al.
Scientific reports, 6(1), 38278-38278 (2016)
Nousheen Zaidi et al.
Biochemical and biophysical research communications, 376(1), 5-9 (2008-09-03)
Cathepsin D is a normal and major component of lysosomes, it is found in almost all cells and tissues of mammals. Present review describes different events in cellular life of cathepsin D mainly its biosynthesis, co-translational and posttranslational modifications, targeting
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持