形式
lyophilized powder
质量水平
比活
≥250 units/mg protein (E1%/280)
分子量
~45 kDa
颜色
white
UniProt登记号
储存温度
−20°C
基因信息
human ... CTSD(1509)
一般描述
组织蛋白酶D是一种天冬氨酸蛋白酶，位于溶酶体中。它可参与蛋白质分解代谢并维持激素和抗原的加工过程。组织蛋白酶D与肿瘤形成和神经退行性改变有关。它可调节溶酶体蛋白水解和内源性纤维蛋白溶解。
应用
来自人肝脏的组织蛋白酶D已被用于：
- β分泌酶活性测定
- 酶降解淀粉样β1-42
- 人包皮成纤维细胞的显微注射
生化/生理作用
组织蛋白酶D是一种内体-溶酶体天冬氨酸蛋白酶，与乳腺癌转移和阿尔茨海默病有关。在细胞凋亡的人包皮成纤维细胞中，发现组织蛋白酶D的溶酶体释放先于细胞色素c的释放和膜电位的丧失。诱导凋亡后12至24小时，PC12细胞中的组织蛋白酶D水平升高。
其他说明
等电聚焦观察到含有α、β和γ同工酶。
单位定义
在pH3.3，37°C条件下，一单位可在30分钟内导致A280 增加1.0个单位，该活性利用酸变性血红蛋白作为底物，通过TCA可溶产物进行测定（1 cm光路）。
外形
含磷酸钠缓冲盐的粉末。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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