生物来源
bovine spleen
质量水平
检测方案
10—70% protein (biuret)
形式
lyophilized powder
比活
≥2.0 units/mg protein
分子量
~45 kDa
制造商/商品名称
Sigma-Aldrich
技术
activity assay: suitable
颜色
dark brown
适用性
suitable for molecular biology
UniProt登记号
应用
life science and biopharma
储存温度
−20°C
基因信息
cow ... CTSD(282883)
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一般描述
Research area: Cell Signaling
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
应用
Cathepsin D from bovine spleen has been used:
- in in vitro dose-dependent fluorometric activity assays.
- in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
- for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
- in cathepsin D activity assay.
生化/生理作用
组织蛋白酶D是一种核内体-溶酶体天冬氨酸蛋白酶，与乳腺癌转移和阿尔茨海默病有关。 人们发现，在凋亡的人包皮成纤维细胞中，溶酶体释放组织蛋白酶D先于释放细胞色素c和膜电位丧失。 诱导凋亡后12至24小时，PC12细胞中的组织蛋白酶D水平升高。
单位定义
在pH 3.0、37 °C条件下，一个单位可在一分钟内使一毫升溶液的A280增加1.0（以血红蛋白为底物，对TCA可溶性产物进行测定（1 cm光程））。
外形
含有柠檬酸盐缓冲液盐的冻干粉
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Martin Stolz et al.
Biophysical journal, 86(5), 3269-3283 (2004-04-28)
Cartilage stiffness was measured ex vivo at the micrometer and nanometer scales to explore structure-mechanical property relationships at smaller scales than has been done previously. A method was developed to measure the dynamic elastic modulus, |E(*)|, in compression by indentation-type
Olja Mijanovic et al.
Pharmaceutics, 13(6) (2021-07-03)
Lysosomal proteases play a crucial role in maintaining cell homeostasis. Human cathepsin D manages protein turnover degrading misfolded and aggregated proteins and favors apoptosis in the case of proteostasis disruption. However, when cathepsin D regulation is affected, it can contribute
Marie Maynadier et al.
Journal of controlled release : official journal of the Controlled Release Society, 171(2), 251-257 (2013-08-01)
Implication of the intracellular proteolytic activity of Cathepsin D (CathD), a lysosomal aspartyl-protease overexpressed in numerous solid tumors, has been evidenced on tumor growth. Its intracellular inhibition by potent inhibitors such as pepstatin constitutes a relevant but challenging molecular target.
Yoko Shiba et al.
Current biology : CB, 23(19), 1945-1951 (2013-10-01)
ArfGAPs are known to be involved in cargo sorting in COPI transport. However, the role of ArfGAPs in post-Golgi membrane traffic has not been defined. To determine the function of ArfGAPs in post-Golgi traffic, we used small interfering RNA to
Cathepsin D from porcine and bovine spleen.
T Takahashi et al.
Methods in enzymology, 80 Pt C, 565-581 (1981-01-01)
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