组织蛋白酶B，来源于人肝脏

Research area: Cell Signaling

Native cathepsin B from human liver, purified by affinity chromatography and HPLC. The most investigated enzyme of all lysosomal cysteine proteases. Cathepsin B belongs to the papain-like family of cysteine proteases and is produced as a preproenzyme. It is a bilobal protein, and its catalytic site is situated at the interface between the two lobes.

Cathepsin B, Human Liver, has been used in:

• Diagnostics: as a potent and independent prognostic marker for endometrial cancer, pancreatic adenocarcinoma, and inflammatory disease.
• Drug development: during the neovascularization process and as a potent therapeutic target for various pathologies, cancer progression, and osteoarthritis in humans.
• Pharmacology: for increasing the therapeutic index of doxorubicin by incorporating the cathepsin B cleavable spacer Phe-Lys-4-aminobenzyloxycarbonyl into an albumin-binding prodrug.
• Molecular biology: in cathepsin B activity assay.

Cathepsin B acts as both endo and exopeptidase. While as an endopeptidase it cleaves amino acids with a large hydrophobic side chain in the P2 site of the protein/peptide substrate, on the other hand as an exopeptidase it eliminates two amino acids (dipeptide) from the C terminus of a polypeptide substrate, thereby categorizing the enzyme as a peptidyl dipeptidase. It maintains homeostatic metabolic activity within cells through regular turnover of both intracellular and extracellular proteins. Additionally, it is involved in various cellular functions like regulation of pro-enzyme and pro-hormone activation, tissue remodeling, antigen processing, apoptosis, and inflammatory responses to antigens. The expression and activity of cathepsin B have been linked to several pathologies, including cardiovascular disease, cancer, Alzheimer’s, arthritis, and pancreatitis. Overexpression of cathepsin B has been observed in brain, breast, gastric, prostate, esophageal, skin, lung, ovarian, colon, and thyroid cancers and correlates positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

毒性：标准处理（A）

一个单位定义为使用100 mM Na⁺/K⁺（pH值6.0），1.33 mM EDTA和2 mM DTT作为活化缓冲液，在40°C下每分钟水解1.0 µmol Z-RR-β-萘酰胺的酶量。

在50 mM醋酸钠缓冲液、1 mM EDTA（pH 5.0）中。

由已经认证检测显示对HBsAg以及HIV和HCV抗体呈阴性的个体组织而制备。

初次解冻后，分装冻存（-70°C）。

Hirai, K., et al. 1999.Hum.Pathol.30, 680.
Kostoulas, G., et al. 1999.FEBS Lett.455, 286.
Strojnik, T., et al. 1999.Clin. Cancer Res.5, 559.
Maquire, T.M., et al. 1998.Int. J. Biol. Markers13, 139.
Berquim, I.M., and Sloane, B.F.1996.Adv.Exp.Med. Biol.389, 281.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany