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一般描述
Gel filtration chromatography is a well-known technique for measuring the size and molecular weight of proteins. It separates proteins based on their ability to diffuse into the pores of the resin. Larger proteins have more difficulty entering the resin pores, so they flow through the column more quickly than smaller proteins. As a result, the proteins are eluted from the column in order of decreasing molecular weight.
应用
凝胶过滤标记物试剂盒（蛋白分子量29,000-700,000 Da）用于：
- 蛋白纯化和分析（凝胶过滤实验）
- 突触神经小体提取物的体积排阻色谱/凝胶过滤色谱
- 甘油梯度离心
- 研究糖皮质诱导淋巴细胞溶解，作为免疫系统中细胞凋亡的模式体系
- 分离玉竹凝集素，其对Vero细胞具有显著的抗HSV-II活性，对A375人黑素瘤细胞具有细胞毒性，并可以半胱天冬酶依赖性方式诱导细胞凋亡
生化/生理作用
The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.
其他说明
- For research use only. Not for drug, household, or other uses.
- Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
- Please refer to the product information sheet to learn more about reagents, storage and stability, and procedure.
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- A8531Albumin, bovine serum, 66,000 MW 1 mL/vial化学品安全说明书
- A8656Alcohol Dehydrogenase, yeast, 150,000 MW 1 mL/vial化学品安全说明书
- A8781 β-Amylase, sweet potato, 200,000 MW 1 mL/vial化学品安全说明书
- A3660 Apoferritin, horse spleen, 443,000 MW 1 mL/vial化学品安全说明书
- D4772Blue dextran, 2,000,000 MW 1 mL/vial化学品安全说明书
- C7025Carbonic Anhydrase, bovine erythrocytes, 29,000 MW 1 mL/vial化学品安全说明书
- T9145Thyroglobulin, bovine, 669,000 MW 1 mL/vial化学品安全说明书
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Germán L Rosano et al.
The Journal of biological chemistry, 286(34), 29671-29680 (2011-07-09)
HSP100 proteins are molecular chaperones involved in protein quality control. They assist in protein (un)folding, prevent aggregation, and are thought to participate in precursor translocation across membranes. Caseinolytic proteins ClpC and ClpD from plant chloroplasts belong to the HSP100 family.
D K Wimsatt et al.
Clinical chemistry, 33(11), 2100-2106 (1987-11-01)
The Coomassie Brilliant Blue G-250 method for urinary proteins underestimates urinary immunoglobulin light chains when albumin or pooled serum is used as the protein standard. The specific color yields of these and other proteins can be brought closer together by
Lei Yue et al.
Nucleic acids research, 48(17), 9589-9605 (2020-08-29)
Transcription termination defines accurate transcript 3'-ends and ensures programmed transcriptomes, making it critical to life. However, transcription termination mechanisms remain largely unknown in Archaea. Here, we reported the physiological significance of the newly identified general transcription termination factor of Archaea
Qi Zong Lao et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(12), 5013-5023 (2010-08-25)
Voltage-gated calcium channels conduct Ca(2+) ions in response to membrane depolarization. The resulting transient increase in cytoplasmic free calcium concentration is a critical trigger for the initiation of such vital responses as muscle contraction and transcription. L-type Ca(v)1.2 calcium channels
Thorsten Gantke et al.
The Biochemical journal, 452(2), 359-365 (2013-04-06)
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of
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