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生物来源
bovine
质量水平
形式
powder
分子量
~66 kDa
~66,000
包装
vial of 50 mg
来源
USA origin
技术
ELISA: suitable
gel permeation chromatography (GPC): suitable
immunohistochemistry: suitable
杂质
~0.3% dithiothreitol
pH值（酸碱度）
<5.0
适用性
suitable for (Suitable for use as a gel filtration marker)
UniProt登记号
异质活性
BT virus, none detected
VSV virus, none detected
储存温度
−20°C
基因信息
bovine ... ALB(280717)
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一般描述
Albumin is the most abundant plasma protein in humans. It has a molecular mass of 66.5 kDa.
应用
Albumin from bovine serum has been used:
- for blocking of nonspecific immunoglobulin during immunohistochemical reactions.
- to coat microplates for blocking non-specific binding during I-ELISA (Indirect Enzyme-linked Immunosorbent Assay)
- as molecular mass standard in analytical gel filtration chromatography for Superdex® 75 10/300 GL gel filtration column.
- for measuring fluorescence for PR1 (phytofluor red 1) by using Fluorescence correlation spectroscopy (FCS)
- as a reference mixture along with horse Mb in Size Exclusion Chromatography
Gel filtration molecular weight marker
生化/生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also triggers the insulin dependent diabetes mellitus.
High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Albumin in serum level is associated with advanced liver disease.
制备说明
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
法律信息
Superdex is a registered trademark of Cytiva
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Zhongmei Liu et al.
Biochemistry, 49(20), 4349-4360 (2010-04-16)
Caenorhabditis elegans gelsolin-like protein-1 (GSNL-1) is a new member of the gelsolin family of actin regulatory proteins [Klaavuniemi, T., Yamashiro, S., and Ono, S. (2008) J. Biol. Chem. 283, 26071-26080]. It is an unconventional gelsolin-related protein with four gelsolin-like (G)
Abigail E Miller et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(30), 11136-11141 (2006-07-18)
Fluorescence correlation spectroscopy (FCS) was used to investigate the hydrodynamic and photophysical properties of PR1 (phytofluor red 1), an intensely red fluorescent biliprotein variant of the truncated cyanobacterial phytochrome 1 (Cph1Delta, which consists of the N-terminal 514 amino acids). Single-molecule
UCAN US
Rev. Med. Vet. (Toulouse), 161(2), 51-56 (2010)
J C Noujaim et al.
Poultry science, 87(5), 927-933 (2008-04-19)
The expression of immune response in the form of leukocytic infiltrate by CD3+, CD4+, and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks was studied in the present work by means of immunohistochemical reaction. The
Y Qiu et al.
The Journal of biological chemistry, 273(36), 23426-23432 (1998-08-29)
Myoglobin (Mb) has been believed to be absent generally from mammalian smooth muscle tissue. Examination of human rectal, uterine, bladder, colon, small intestine, arterial, and venous smooth muscle by immunohistochemical techniques shows that each of these tissues is immunopositive for
实验方案
Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.
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