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Merck
CN

A8781

β-淀粉酶 来源于甘薯

greener alternative

别名:

1,4-α-D-葡聚糖麦芽水解酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
41106514
NACRES:
NA.56
EC Number:
232-566-1
MDL number:
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form

powder

mol wt

~200,000

packaging

vial of 15 mg

greener alternative product score

old score: 57
new score: 1
Find out more about DOZN™ Scoring

greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

storage temp.

−20°C

Quality Level

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Application

β-来自甘薯的淀粉酶已被用于:
  • 作为蓝绿温和胶电泳(PAGE)中的分子量标记
  • 作为尺寸排阻色谱中制定校准曲线的标准品
  • 作为小角度X射线分析(SAXs)的模型蛋白

Biochem/physiol Actions

β-淀粉酶作用于多糖α(1,4)糖苷键并释放非还原末端的麦芽糖。凭借高稳定性,来自甘薯的β-淀粉酶可与琼脂糖微球一起用于固定化酶制剂中。

General description

β-来自甘薯的淀粉酶是一种四聚体酶,每个单体包含(α/β)核心结构域和C-末端环区(loop region)。它属于GH14糖苷水解酶家族。
我们竭诚为您带来满足绿色替代产品四大类别要求的替代产品。本品属于重新设计产品类别,在“防止废弃物”、“更安全的溶剂和助剂”以及“节能设计”绿色化学原则方面取得了重大改进。 点击此处查看其 DOZN 记分卡。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Purification of staphylococcal protein A by affinity precipitation using a reversibly soluble-insoluble polymer with human IgG as a ligand
Taniguchi, M. et al.
Journal of Fermentation and Bioengineering, 68, 32-36 (1989)
Determination of hydrodynamic radius of proteins by size exclusion chromatography
LaVV, et al.
Bio-protocol, 7(8), 14-Jan-14-Jan (2017)
Luca Costa et al.
BMC structural biology, 16(1), 18-18 (2016-10-30)
Synchrotron radiation facilities are pillars of modern structural biology. Small-Angle X-ray scattering performed at synchrotron sources is often used to characterize the shape of biological macromolecules. A major challenge with high-energy X-ray beam on such macromolecules is the perturbation of
Khalilah G Reddie et al.
Molecular bioSystems, 4(6), 521-531 (2008-05-22)
Oxidation of the thiol functional group in cysteine (Cys-SH) to sulfenic (Cys-SOH), sulfinic (Cys-SO2H) and sulfonic acids (Cys-SO3H) is emerging as an important post-translational modification that can activate or deactivate the function of many proteins. Changes in thiol oxidation state
Optimization of the immobilization of sweet potato amylase using glutaraldehyde-agarose support. Characterization of the immobilized enzyme
Tavano OL, et al.
Process. Biochem., 48(7), 1054-1058 (2013)

实验方案

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.

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