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Merck
CN

A8781

Sigma-Aldrich

β-淀粉酶 来源于甘薯

greener alternative

别名:

1,4-α-D-葡聚糖麦芽水解酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
41106514
NACRES:
NA.56

表单

powder

质量水平

分子量

~200,000

包装

vial of 15 mg

环保替代产品得分

old score: 57
new score: 1
Find out more about DOZN™ Scoring

环保替代产品特性

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

环保替代产品分类

储存温度

−20°C

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一般描述

β-来自甘薯的淀粉酶是一种四聚体酶,每个单体包含(α/β)核心结构域和C-末端环区(loop region)。它属于GH14糖苷水解酶家族。
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应用

β-来自甘薯的淀粉酶已被用于:
  • 作为蓝绿温和胶电泳(PAGE)中的分子量标记
  • 作为尺寸排阻色谱中制定校准曲线的标准品
  • 作为小角度X射线分析(SAXs)的模型蛋白

生化/生理作用

β-淀粉酶作用于多糖α(1,4)糖苷键并释放非还原末端的麦芽糖。凭借高稳定性,来自甘薯的β-淀粉酶可与琼脂糖微球一起用于固定化酶制剂中。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

动植物源性产品
常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

S Teotia et al.
Enzyme and microbial technology, 28(9-10), 792-795 (2001-06-09)
beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band
Integration of affinity precipitation with partitioning methods for bioseparation of chitin binding lectins.
Teotia, S. et al.
Food and Bioproducts Processing, 84, 37-43 (2006)
Liyu Tu et al.
Journal of cell science, 119(Pt 24), 5077-5086 (2006-12-13)
The surface of the mammalian urinary bladder is covered by a crystalline, asymmetric unit membrane (AUM) structure that contains the four major uroplakins (UPs): Ia, Ib, II and IIIa. UPIa and UPIb belong to the family of tetraspanins. Although UPIa
Determination of hydrodynamic radius of proteins by size exclusion chromatography
LaVV, et al.
Bio-protocol, 7(8), 14-Jan-14-Jan (2017)
Purification of staphylococcal protein A by affinity precipitation using a reversibly soluble-insoluble polymer with human IgG as a ligand
Taniguchi, M. et al.
Journal of Fermentation and Bioengineering, 68, 32-36 (1989)

实验方案

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.

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