P6236
焦谷氨酸氨肽酶 来源于激烈热球菌
recombinant, expressed in E. coli, ~90% (SDS-PAGE), ≥5.0 units/mg protein
别名:
L-吡咯烷酮羧基肽酶
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100 ML
¥211.05
500 ML
¥665.04
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¥2,434.10
24 X 500 ML
¥11,932.77
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国内现货，预计发货时间2025年4月14日详情
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100 ML
¥211.05
500 ML
¥665.04
6 X 500 ML
¥2,434.10
24 X 500 ML
¥11,932.77
About This Item
¥211.05
国内现货，预计发货时间2025年4月14日详情
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此商品 | PHR1419 | V900220 | 100684 |
|---|---|---|---|
| shelf life limited shelf life, expiry date on the label | shelf life - | shelf life - | shelf life - |
| composition acetic acid (30%), 1000 mL | composition - | composition - | composition - |
| technique(s) microbe id | metabolite detection: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) - | technique(s) - |
| application(s) agriculture | application(s) pharmaceutical (small molecule) | application(s) - | application(s) - |
| suitability Enterobacter spp. | suitability - | suitability - | suitability - |
一般描述
来自 强烈火球菌的焦谷氨酸氨基酞酶也被称为解封闭氨肽酶，是一种42kDa的蛋白质，属于氨肽酶A家族。它与活性位点中的氨肽酶具有序列同源性，具有保守的锌和钴结合残基。[1]
应用
在Edman降解之前，从蛋白质和肽中释放N-末端焦谷氨酸的热稳定氨肽酶。
在纯化的cassiicolin的N-末端测序之前，来自Sigma的酶已被用于在还原条件下除去焦谷氨酸（pGlu）N-末端封闭基团。[2]
来自 强烈火球菌的焦谷氨酸氨基酞酶是一种重组的、热稳定的氨肽酶，其表达于 大肠杆菌。它可用于切割焦谷氨酸，从而对肽的N-末端序列进行分析。
生化/生理作用
该酶对N-末端焦谷氨酸具有特异性。它在Edman降解之前，可从蛋白质和肽中切割N-末端焦谷氨酸。其最适温度范围为95至100°C，最适pH范围为6.0至9.0。
单位定义
在pH 7.0，37 °C条件下，一个单位每分钟可水解1 μmol焦谷氨酸对硝基苯胺。
外形
含有磷酸钠的冻干粉
制备说明
用含有10mM DTT和1mM EDTA的50 μl 50mM磷酸钠（pH 7.0）溶液重溶瓶中的酶。重溶的溶液应储存在-20°C。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
New deblocking aminopeptidases from Pyrococcus horikoshii
Mori K and Ishikawa K
Bioscience, Biotechnology, and Biochemistry, 69(10), 1854-1860 (2005)
Pyroglutamate aminopeptidase 1 may be an indicator of cellular inflammatory response as revealed using a sensitive long-wavelength fluorescent probe
Gong Q, et al.
Chemical Science, 7(7), 4694-4697 (2016)
A Ultrasensitive Near-Infrared Fluorescent Probe Reveals Pyroglutamate Aminopeptidase 1 Can Be a New Inflammatory Cytokine
Gong Q, et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 5(4), 1700664-1700664 (2018)
Frédéric de Lamotte et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 849(1-2), 357-362 (2006-11-23)
Cassiicolin, a phytotoxin produced by the necrotrophic fungus Corynespora cassiicola, was purified to homogeneity from a rubber tree isolate. The optimized protocol involves reverse phase chromatography followed by size exclusion chromatography, with monitoring of the toxicity on detached rubber tree
An Staes et al.
Proteomics, 8(7), 1362-1370 (2008-03-05)
We previously described a proteome-wide, peptide-centric procedure for sorting protein N-terminal peptides and used these peptides as readouts for protease degradome and xenoproteome studies. This procedure is part of a repertoire of gel-free techniques known as COmbined FRActional DIagonal Chromatography
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