一般描述
该酶在pH 8-10下稳定，可在24小时保存高达90%的活性。其55-60℃下具有最大活性。
特异性
枯草素A 是丝氨酸S8内蛋白酶家族的一个成员。它具有广泛的特异性，在P1位置有较大的非荷电残基。它可水解天然和变性蛋白，并且在碱性条件下具有活性。
应用
该产品已与其他酶一起用于 原位 蛋白水解，以制备适用于结构测定的晶体。它还被用于分离可用于 体外 功能研究的肌膜下(SS)和肌原纤维间(IMF)线粒体。
这是由 衣芽孢杆菌发酵而分离出来的一种蛋白水解酶。这是一种丝氨酸内蛋白酶，对天然和变性蛋白具有广泛的特异性，在碱性条件下具有活性。
生化/生理作用
蛋白酶通过水解肽键分解蛋白质。 蛋白酶可被丝氨酸活性位点抑制剂灭活，如苯基甲基磺酰氟(PMSF)和二异丙基氟磷酸酯。 蛋白酶，P5380的产物，是一种丝氨酸内蛋白酶，对天然和变性蛋白具有广泛的特异性，在碱性条件下具有活性。 它在某些有机溶剂中具有活性，如干辛烷。
物理属性
枯草素 是一种无二硫键的非糖基化单肽链，其分子量为27kda。
单位定义
一单位在37°C、pH 7.5条件下每分钟可水解1.0μmole(181 μg)的酪氨酸（福林酚试剂显色）。
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
Tommi Kotila et al.
Nature communications, 9(1), 1892-1892 (2018-05-16)
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Carley R Benton et al.
The Journal of physiology, 586(6), 1755-1766 (2008-02-02)
Peroxisome proliferator-activated receptors (PPARs) alter the expression of genes involved in regulating lipid metabolism. Rosiglitazone, a PPARgamma agonist, induces tissue-specific effects on lipid metabolism; however, its mode of action in skeletal muscle remains unclear. Since fatty acid translocase (FAT/CD36) was
Marie Deprez et al.
American journal of respiratory and critical care medicine, 202(12), 1636-1645 (2020-07-30)
Rationale: The respiratory tract constitutes an elaborate line of defense that is based on a unique cellular ecosystem.Objectives: We aimed to investigate cell population distributions and transcriptional changes along the airways by using single-cell RNA profiling.Methods: We have explored the
Sarah E Ehrlicher et al.
Frontiers in endocrinology, 12, 651211-651211 (2021-04-20)
Lipid overload of the mitochondria is linked to the development of insulin resistance in skeletal muscle which may be a contributing factor to the progression of type 2 diabetes during obesity. The targeted degradation of mitochondria through autophagy, termed mitophagy
L Proctor et al.
Gynecologic oncology, 146(3), 596-602 (2017-06-26)
We sought to determine whether DNA ploidy correlates with the four molecular subgroups of endometrial carcinoma (EC) as determined using ProMisE (Proactive Molecular Risk Classifier for Endometrial Cancer). 90 cases of EC previously characterized by clinicopathological parameters, outcomes, and ProMisE
实验方案
本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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