特异性
应用
- 消化髓核（NP）组织
- 催化不对称曼尼希一锅反应
- 淀粉脱蛋白
- 消化细胞解离用脑切片
生化/生理作用
特点和优势
- 在pH 5.0中9.0范围内高度稳定，在pH 8.8具有最高活性
- 与多种DNA和RNA分离缓冲液兼容
- 广泛的底物特异性
质量
物理属性
单位定义
制备说明
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
Is Product P5147, Protease from Streptomyces griseus, also described as "Pronase"?
"Pronase E" is the name given to a group of proteolytic enzymes produced by Streptomyces griseus K-1. At least 10 proteases are in the mixture, five serine-type proteases, two zinc endopeptidases, two zinc leucine aminopeptidases, and one zinc carboxypeptidase.
Does Product P5147, Protease from Streptomyces griseus, contain calcium acetate?
This product contains calcium acetate buffer salt. The amount of calcium acetate present will vary for each lot number and has been observed to range from 2 - 30% (w/w).
How should Product P5147, Protease from Streptomyces griseus, be reconstituted and how should a stock solution be prepared?
This product can be dissolved in 0.01 M sodium acetate with 0.005 M calcium acetate at pH 7.5 at 37°C; at 0.2 mg/mL, this yields a clear solution which ranges from colorless to light tan. Calcium ion is recommended for protection from autolysis. The activity of a dilute enzyme solution containing 0.01 to 0.1 M calcium ion was stable over 24 hours at neutral pH at 2-8°C. Pronase E is stable at 4°C for at least six months. Stock solutions of 5 to 20 mg/mL in water are usually stored at -20°C.
Under what conditions can Product P5147, Protease from Streptomyces griseus, be used to digest protein?
For DNA isolation, Pronase E is usually prepared as a stock solution, and prior to storage at -20°C, the solution is first heated to 56°C for about 15 minutes, then incubated at 37°C for 1 hour. This encourages self-digestion, to eliminate DNAse and RNAse contamination. The enzyme is added to a DNA sample (in the presence of 0.5-1%(w/v) SDS to disrupt DNA protein interactions) typically at 250-500 μg protein/mL, then incubated at 37°C for 1-4 hours. For protein hydrolysis, dissolve about 0.2 μmol of protein in 0.2 mL of 50 mM ammonium bicarbonate buffer at pH 8 (or phosphate buffer pH 7). Add Pronase to 1% (w/w) and incubate at 37°C for 24 hours. It may be necessary to add aminopeptidase M at 4% (w/w) and incubate at 37°C for another 18 hours.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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