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安全信息

P2893

Sigma-Aldrich

JumpStart™ Taq ReadyMix™

Complete optimized reagent for hot-start PCR at 2X concentration

别名:

hot start PCR master mix, hot start master mix, hot start taq master mix, mutiplex PCR

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About This Item

MDL编号:
MFCD00166026
UNSPSC代码:
41106300
NACRES:
NA.55

质量水平

200

形式

liquid

用途

sufficient for 100 reactions
 sufficient for 400 reactions

特点

Multiplex PCR
dNTPs included
hotstart

浓度

2.5 units/reaction (50 μL reaction volume)

技术

PCR: suitable

颜色

colorless

适用性

suitable for (quantitative PCR)

应用

agriculture

运输

wet ice

储存温度

−20°C

相关类别

一般描述

JumpStart™ Taq ReadyMix™是一种即用型2X预混液，其中含有JumpStart™ Taq DNA聚合酶、99％纯dNTP、反应缓冲液和JumpStart™抗体。Taq DNA聚合酶是一种抗体灭活的热启动酶。一旦反应温度达到70°C，DNA聚合酶-抗体复合物随即解离，Taq DNA聚合酶活性得以恢复。相对于手动热启动技术，这种抗体-酶复合物可轻松简便地准备，同时可降低污染风险。

应用

JumpStart™ Taq ReadyMix™ 已用于定量聚合酶链式反应(PCR)。此产品还可用于鉴定缺氧条件下的成纤维细胞表面腺苷受体(AR)类型。同时适用于：
  • 用于需要减少非特异性扩增的 PCR 扩增
  • 用于多重 PCR
  • 用于减少引物二聚体

特点和优势

  • 该预混液可确保反应间一致性
  • 缩短了准备时间，并减少了多个移液步骤造成的污染风险
  • 可最大限度减少非特异性扩增和污染
  • 提高了特异性和靶标产量
  • 减少了引物二聚体
  • 与手动或蜡质热启动方法相比，准备时间更短
  • 允许室温反应
  • 非常适合高通量定量PCR应用

包装

默认反应体积为50 μL

100个反应包装为1×2.5mL
400个反应包装为1×10mL

其他说明

JumpStart Taq ReadyMix是一种制备好的溶液，其结合了热启动PCR的性能优势和ReadyMix的便利性。该混合物包括JumpStart Taq DNA聚合酶、99％纯脱氧核苷酸、以及2x优化的反应浓缩液缓冲液。JumpStart Taq聚合酶是一种抗体灭活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量。由于JumpStart Taq聚合酶的聚合酶活性在PCR反应的第一个变性循环期间即可完全恢复，因而其与其他热启动方法（即化学灭活）不同，在循环前不需要预孵育步骤。热启动机制便于在室温进行设置，使得其成为高通量应用的理想选择。若需要制备50 μL反应，只需将25 μL ReadyMix添加至模板、引物和水中，最终反应体积为50 μL。
2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
准备50 μL反应物，只需将 25 μL ReadyMix™加入模板、引物和水，至终反应体积50 μL。
本品已通过定量PCR验证，但可能需要补充25 mM氯化镁溶液（货号M8787）、适宜的荧光探针以及（如果需要）内参染料（货号R4526）。请在www.sigma-aldrich.com/hotstart页面查看有关JumpStart Taq ReadyMix产品的更多详细信息。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：美国：US 8,404,464和US 7,972,828。 根据前述专利权利要求，购买本产品包含有限不可转让的免于诉讼豁免。
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
含少量动物源组分生物产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the fidelity of Taq DNA Polymerase?

    The error rates for polymerases are more accurately presented in terms of ranges since the actual fidelity is condition dependent. The error rate of standard Taq, as reported in the literature, is in a range of 1 in 1.1 × 104 (Tindall, K.R., and Kunkel, T.A., Biochemistry, 27, 6008-6013 (1988)) to 1 in 2.0 × 105 (Lundberg, K.S., Shoemaker, D.D., Adams, M.W., Short, J.M., Sorge, J.A.and Mathur, E.J., Gene, 108(1), 1-6 (1991)). These values would apply to this product.

  6. Can Product P2893, JumpStart™ Taq ReadyMix™, be used in real-time PCR?

    Yes.  The mixture does not contain any internal reference dye, though.  D7440 comes with a vial of our internal reference dye, R4526, which can also be purchased separately.

  7. Can PCR products made with Product P2893, JumpStart™ Taq ReadyMix™, be used for TA cloning?

    Yes, this enzyme does not have 3'-5' exonuclease activity, so there will be 3'A overhangs available for this type of cloning approach.

  8. Can Product P2893, JumpStart™ Taq ReadyMix™, be used with Bio-Rad real-time PCR machines?

    Yes.  Bio-Rad's real-time instruments typically require fluorescein as an internal reference dye. We have fluorescein as product F6377 (Fluorescein, sodium salt).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Valerie F Boltz et al.
Retrovirology, 13(1), 87-87 (2016-12-22)
Although next generation sequencing (NGS) offers the potential for studying virus populations in unprecedented depth, PCR error, amplification bias and recombination during library construction have limited its use to population sequencing and measurements of unlinked allele frequencies. Here we report
S Kwok et al.
Nature, 339(6221), 237-238 (1989-05-18)
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.
Diego Ottaviani et al.
Genome research, 18(11), 1778-1786 (2008-10-14)
The folding of chromatin into topologically constrained loop domains is essential for genomic function. We have identified genomic anchors that define the organization of chromatin loop domains across the human major histocompatibility complex (MHC). This locus contains critical genes for
Alex J McCarthy et al.
Infection and immunity, 87(5) (2019-03-06)
Gastrointestinal (GI) colonization of 2-day-old (P2) rat pups with Escherichia coli K1 results in translocation of the colonizing bacteria across the small intestine, bacteremia, and invasion of the meninges, with animals frequently succumbing to lethal infection. Infection, but not colonization
Koen Kole et al.
GigaScience, 6(10), 1-6 (2017-10-13)
Experience-dependent plasticity (EDP) is essential for anatomical and functional maturation of sensory circuits during development. Although the principal synaptic and circuit mechanisms of EDP are increasingly well studied experimentally and computationally, its molecular mechanisms remain largely elusive. EDP can be
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Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

终点 PCR：特异性和产量增强的抗体介导的热启动 PCR 方案

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