跳转至内容
Merck
CN

P2893

JumpStart™ Taq ReadyMix™

Complete optimized reagent for hot-start PCR at 2X concentration

别名:

hot start PCR master mix, hot start master mix, hot start taq master mix, mutiplex PCR

登录 查看组织和合同定价。

选择尺寸

关于此项目

UNSPSC Code:
41106300
NACRES:
NA.55
MDL number:
MFCD00166026

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

JumpStart™ Taq ReadyMix™, Complete optimized reagent for hot-start PCR at 2X concentration

feature

hotstart
Multiplex PCR
dNTPs included

form

liquid

usage

sufficient for 100 reactions
 sufficient for 400 reactions

concentration

2.5 units/reaction (50 μL reaction volume)

technique(s)

PCR: suitable

color

colorless

suitability

suitable for (quantitative PCR)

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

相关类别

Application

JumpStart™ Taq ReadyMix™ 已用于定量聚合酶链式反应(PCR)。此产品还可用于鉴定缺氧条件下的成纤维细胞表面腺苷受体(AR)类型。同时适用于：
  • 用于需要减少非特异性扩增的 PCR 扩增
  • 用于多重 PCR
  • 用于减少引物二聚体

Features and Benefits

  • 该预混液可确保反应间一致性
  • 缩短了准备时间，并减少了多个移液步骤造成的污染风险
  • 可最大限度减少非特异性扩增和污染
  • 提高了特异性和靶标产量
  • 减少了引物二聚体
  • 与手动或蜡质热启动方法相比，准备时间更短
  • 允许室温反应
  • 非常适合高通量定量PCR应用

General description

JumpStart™ Taq ReadyMix™是一种即用型2X预混液，其中含有JumpStart™ Taq DNA聚合酶、99％纯dNTP、反应缓冲液和JumpStart™抗体。Taq DNA聚合酶是一种抗体灭活的热启动酶。一旦反应温度达到70°C，DNA聚合酶-抗体复合物随即解离，Taq DNA聚合酶活性得以恢复。相对于手动热启动技术，这种抗体-酶复合物可轻松简便地准备，同时可降低污染风险。

Legal Information

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：美国：US 8,404,464和US 7,972,828。 根据前述专利权利要求，购买本产品包含有限不可转让的免于诉讼豁免。
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
JumpStart Taq ReadyMix是一种制备好的溶液，其结合了热启动PCR的性能优势和ReadyMix的便利性。该混合物包括JumpStart Taq DNA聚合酶、99％纯脱氧核苷酸、以及2x优化的反应浓缩液缓冲液。JumpStart Taq聚合酶是一种抗体灭活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量。由于JumpStart Taq聚合酶的聚合酶活性在PCR反应的第一个变性循环期间即可完全恢复，因而其与其他热启动方法（即化学灭活）不同，在循环前不需要预孵育步骤。热启动机制便于在室温进行设置，使得其成为高通量应用的理想选择。若需要制备50 μL反应，只需将25 μL ReadyMix添加至模板、引物和水中，最终反应体积为50 μL。
准备50 μL反应物，只需将 25 μL ReadyMix™加入模板、引物和水，至终反应体积50 μL。
本品已通过定量PCR验证，但可能需要补充25 mM氯化镁溶液（货号M8787）、适宜的荧光探针以及（如果需要）内参染料（货号R4526）。请在www.sigma-aldrich.com/hotstart页面查看有关JumpStart Taq ReadyMix产品的更多详细信息。

Packaging

默认反应体积为50 μL

100个反应包装为1×2.5mL
400个反应包装为1×10mL

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
含少量动物源组分生物产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000472990
0000469514
0000469518
0000469518
0000469514

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the fidelity of Taq DNA Polymerase?

    The error rates for polymerases are more accurately presented in terms of ranges since the actual fidelity is condition dependent. The error rate of standard Taq, as reported in the literature, is in a range of 1 in 1.1 × 104 (Tindall, K.R., and Kunkel, T.A., Biochemistry, 27, 6008-6013 (1988)) to 1 in 2.0 × 105 (Lundberg, K.S., Shoemaker, D.D., Adams, M.W., Short, J.M., Sorge, J.A.and Mathur, E.J., Gene, 108(1), 1-6 (1991)). These values would apply to this product.

  6. Can Product P2893, JumpStart™ Taq ReadyMix™, be used in real-time PCR?

    Yes.  The mixture does not contain any internal reference dye, though.  D7440 comes with a vial of our internal reference dye, R4526, which can also be purchased separately.

  7. Can PCR products made with Product P2893, JumpStart™ Taq ReadyMix™, be used for TA cloning?

    Yes, this enzyme does not have 3'-5' exonuclease activity, so there will be 3'A overhangs available for this type of cloning approach.

  8. Can Product P2893, JumpStart™ Taq ReadyMix™, be used with Bio-Rad real-time PCR machines?

    Yes.  Bio-Rad's real-time instruments typically require fluorescein as an internal reference dye. We have fluorescein as product F6377 (Fluorescein, sodium salt).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Effects of A2BR on the biological behavior of mouse renal fibroblasts during hypoxia
Tang Jin, et al.
Molecular Medicine Reports, 11(6), 4397-4402 (2015)
Koen Kole et al.
GigaScience, 6(10), 1-6 (2017-10-13)
Experience-dependent plasticity (EDP) is essential for anatomical and functional maturation of sensory circuits during development. Although the principal synaptic and circuit mechanisms of EDP are increasingly well studied experimentally and computationally, its molecular mechanisms remain largely elusive. EDP can be
Madhavi A Naik et al.
International journal of surgical pathology, 28(4), 371-381 (2019-12-25)
Cystic neutrophilic granulomatous mastitis (CNGM) is a histologically characterized variant of granulomatous lobular mastitis that is associated with lipophilic Corynebacterium species. It remains a largely underrecognized entity in India. Our aim was to study CNGM in the Asian Indian population
Diego Ottaviani et al.
Genome research, 18(11), 1778-1786 (2008-10-14)
The folding of chromatin into topologically constrained loop domains is essential for genomic function. We have identified genomic anchors that define the organization of chromatin loop domains across the human major histocompatibility complex (MHC). This locus contains critical genes for
S Kwok et al.
Nature, 339(6221), 237-238 (1989-05-18)
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.
查看所有相关文献

商品

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

热启动PCR

热启动PCR的目的在于抑制PCR反应，从而减少非特异性扩增、防止引物二聚体形成并提高产物产量。

查看所有结果

实验方案

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

终点 PCR：特异性和产量增强的抗体介导的热启动 PCR 方案

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.

相关内容

Modern Plant Breeding

Explore technology and reagent portfolios for plant breeding workflows, accelerating the development of new crop varieties.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持