推荐产品
质量水平
形式
liquid
用途
sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 400 reactions
特点
dNTPs included
hotstart
浓度
1.25 units/reaction (50 μL reaction volume)
技术
qPCR: suitable
颜色
colorless
输入
purified DNA
适用性
suitable for PCR
应用
agriculture
相容性
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000
检测方法
SYBR® Green
运输
wet ice
储存温度
−20°C
正在寻找类似产品? 访问 产品对比指南
相关类别
一般描述
应用
- During RNA extraction, reverse transcription and Quantitative PCR
- During analysis of leptin and leptin receptor expression. (PCR and melting curve analyses were performed)
- For tissue RNA Isolation. (PCR and melting curve analyses were performed)
- in real-time PCR (RT-PCR) of reverse-transcribed cDNA template
- For qPCR amplifications using SYBR
- For multiplex PCR
- to prepare the real-time PCR mixture
- in the quantitative analysis of gene expression by real time, quantitative polymerase chain reaction (qPCR)
- to determine the mRNA expression of nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) gene by qPCR
特点和优势
- 通过在ReadyMix中进行SYBR Green检测提供抗体灭活热启动PCR的优势，是高通量应用的理想之选；只需引物和模板即可
- JumpStart™ Taq DNA聚合酶可防止非特异性产物扩增，进而提高效率和靶标得率。
- SYBR® Green JumpStart® Taq ReadyMix适用于基于SYBR的qPCR，采用MgCl2配制或采用独立小瓶包装，便于优化。ReadyMixes与管式和板式仪器兼容
- 该预混液可确保反应间一致性
- 可最大限度减少污染
- 减少了引物二聚体
- 与手动或蜡质热启动®方法相比，制备时间更短
- 允许室温构建反应并随附荧光染料；qPCR应用的理想之选
包装
20RXN包装为1 X 500μL
100RXN包装为1 X 2.5 mL
400RXN包装为1 X 10 mL
500RXN包装为1 X 12.5 mL
其他说明
原理
SYBR Green I是一种常用的荧光DNA结合染料，可结合所有双链DNA，并通过测量整个循环中荧光的增加来监测检测。SYBR Green I最大激活和发射峰值分别在494nm和521nm。 ROX参考染料仪器设定符合定量PCR参考染料测量需要。 在加入一种热启动介导tag聚合酶，JumpStart Taq后，Sigma′s基于SYBR QPCR检测的特异性被增强。
法律信息
相关产品
警示用语：
Warning
危险分类
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
商品
qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
实验方案
我们的SYBR Green qPCR实验方案是一种旨在准确定量基因表达和RT-PCR反应的的方法
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
相关内容
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门