KCQS00
KiCqStart® SYBR® Green qPCR ReadyMix预混液™
For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
别名:
qPCR master mix, sybr green qPCR
选择尺寸
¥3,110.90
选择尺寸
About This Item
¥3,110.90
推荐产品
表单
liquid
用途
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
特点
dNTPs included
hotstart
储存条件
protect from light
技术
qPCR: suitable
颜色
colorless
输入
purified DNA
相容性
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MiniOpticon
for use with Bio-Rad MyiQ
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
检测方法
SYBR® Green
运输
dry ice
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此商品 | KCQS01 | KCQS02 | KCQS07 |
|---|---|---|---|
| technique(s) qPCR: suitable | technique(s) qPCR: suitable | technique(s) qPCR: suitable | technique(s) qPCR: suitable |
| form liquid | form liquid | form liquid | form liquid |
| usage sufficient for 1250 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | usage sufficient for 500 reactions |
| feature dNTPs included | feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| color colorless | color colorless | color colorless | color light blue |
一般描述
高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要，因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前，处于抗体介导的失活状态。
应用
- 基因表达
- DNA定量
- 染色质免疫沉淀(CHiP)
特点和优势
- 只需33分钟即可获得分析结果
- 高效、灵敏的实时PCR结果
- 几乎不需要多少优化，甚至无需优化
组分
包装：
250次反应* = 2 X 1.25 mL试管
1250次反应* = 10 X 1.25 mL试管
5000次反应* = 1 X 50 mL试管
*设一次反应需20uL
其他说明
不同的实时PCR系统采用不同策略来校准荧光信号和校正孔间光学变化。 为特定仪器匹配合适的qPCR试剂至关重要。 KiCqStart SYBR Green qPCR ReadyMix不含内参染料。 兼容仪器：
- Bio-Rad® CFX384™
- Bio-Rad CFX96™
- Bio-Rad MiniOpticon™
- Bio-Rad/MJ Chromo4™
- Bio-Rad/MJ Opticon 2
- Bio-Rad/MJ Opticon™
- Cepheid SmartCycler®
- Eppendorf Mastercycler® ep realplex
- Eppendorf Mastercycler ep realplex2 s
- Illumina Eco qPCR
- Qiagen/Corbett Rotor-Gene® 3000
- Qiagen/Corbett Rotor-Gene 6000
- Qiagen/Corbett Rotor-Gene Q
- Roche LightCycler® 480
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存，可稳定存放1年。为方便使用，也可在+2至+8°C下储存长达6周。解冻后，充分混匀后再使用。不建议反复冻融产品。不过，冻融20次或+20°C存放2个月后，产品没有出现任何性能损失。
法律信息
推荐
相关产品
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
商品
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
传统的PCR完成后，会通过琼脂糖凝胶——最近更常使用的是毛细管电泳系统——来分辨并分析数据。
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
PCR测定指南可指导您全程了解引物验证和其他测定优化因素，确保实现理想的DNA / RNA定量高灵敏度和特异性。
实验方案
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
基因表达数据的分析需要稳定的参考或上样控制。所述参考通常是一个或多个参照基因。
相关内容
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
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