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Merck
CN

KCQS00

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix预混液

For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

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别名:
qPCR master mix, sybr green qPCR
UNSPSC代码:
41106300
NACRES:
NA.55

形式

liquid

用途

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

特点

dNTPs included
hotstart

储存条件

protect from light

技术

qPCR: suitable

颜色

colorless

输入

purified DNA

相容性

for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MiniOpticon
for use with Bio-Rad MyiQ
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480

检测方法

SYBR® Green

运输

dry ice

储存温度

−20°C

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一般描述

KiCqStart SYBR Green qPCR ReadyMix是2倍浓缩的即用型预混液,含有除引物和模板之外的实时定量PCR(qPCR)所需的全部组分。这种专有缓冲液、稳定剂和Taq DNA热启动聚合酶的独特组合使用快速或常规的SYBR Green qPCR循环方案,具有最高的PCR效率、灵敏度、特异性和稳定可靠的荧光信号。

高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要,因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前,处于抗体介导的失活状态。

应用

KiCqStart® SYBR® Green qPCR ReadyMix预混液已用于实时定量PCR反应(qPCR),用于扩增和定量来自人肠道普雷沃菌(Prevotella copri)的基因组DNA。
PCR扩增:
  • 基因表达
  • DNA定量
  • 染色质免疫沉淀(CHiP)

特点和优势

  • 只需33分钟即可获得分析结果
  • 高效、灵敏的实时PCR结果
  • 几乎不需要多少优化,甚至无需优化

组分

2X反应缓冲液含有优化浓度的MgCl2、dNTP(dATP, dCTP, dGTP, dTTP)、KiCqStart Taq DNA聚合酶、SYBR Green染料和稳定剂。

包装:
250次反应* = 2 X 1.25 mL试管
1250次反应* = 10 X 1.25 mL试管
5000次反应* = 1 X 50 mL试管
*设一次反应需20uL

其他说明

储存条件:
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存,可稳定存放1年。为方便使用,也可在+2至+8°C下储存长达6周。解冻后,充分混匀后再使用。不建议反复冻融产品。不过,冻融20次或+20°C存放2个月后,产品没有出现任何性能损失。
仪器兼容性:
不同的实时PCR系统采用不同策略来校准荧光信号和校正孔间光学变化。 为特定仪器匹配合适的qPCR试剂至关重要。 KiCqStart SYBR Green qPCR ReadyMix不含内参染料。 兼容仪器:
  • Bio-Rad® CFX384
  • Bio-Rad CFX96
  • Bio-Rad MiniOpticon
  • Bio-Rad/MJ Chromo4
  • Bio-Rad/MJ Opticon 2
  • Bio-Rad/MJ Opticon
  • Cepheid SmartCycler®
  • Eppendorf Mastercycler® ep realplex
  • Eppendorf Mastercycler ep realplex2 s
  • Illumina Eco qPCR
  • Qiagen/Corbett Rotor-Gene® 3000
  • Qiagen/Corbett Rotor-Gene 6000
  • Qiagen/Corbett Rotor-Gene Q
  • Roche LightCycler® 480

法律信息

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Chromo4 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
LightCycler is a registered trademark of Roche
Mastercycler is a registered trademark of Eppendorf AG
MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.
Opticon is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SYBR is a registered trademark of Life Technologies
SmartCycler is a registered trademark of Cepheid, Inc.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
常规特殊物品

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Muthappa Senthil-Kumar et al.
Nature protocols, 9(7), 1549-1562 (2014-06-06)
Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) is widely used in various plant species to downregulate the expression of a target plant gene. TRV is a bipartite, positive-strand RNA virus with the TRV1 and TRV2 genomes. To induce post-transcriptional
Tsion Zewdu Minas et al.
Oncotarget, 8(21), 34141-34163 (2016-05-19)
Ewing sarcoma (ES) involves a tumor-specific chromosomal translocation that produces the EWS-FLI1 protein, which is required for the growth of ES cells both in vitro and in vivo. However, an EWS-FLI1-driven transgenic mouse model is not currently available. Here, we
YK-4-279 effectively antagonizes EWS-FLI1 induced leukemia in a transgenic mouse model.
Minas TZ
Oncotarget, 6(35), 37678-37694 (2015)
NLRX1 Enhances Glutamate Uptake and Inhibits Glutamate Release by Astrocytes
Mahmoud S, et al.
Cells, 8(5), 400-400 (2019)
Phebe Verbrugghe et al.
BMC microbiology, 21(1), 23-23 (2021-01-13)
Since its discovery in 2007, the importance of the human gut bacterium Prevotella copri (P. copri) has been widely recognized with its links to diet and health status and potential as next generation probiotic. Therefore, precise, convenient and cost-effective diagnostic

商品

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

传统的PCR完成后,会通过琼脂糖凝胶——最近更常使用的是毛细管电泳系统——来分辨并分析数据。

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

PCR测定指南可指导您全程了解引物验证和其他测定优化因素,确保实现理想的DNA / RNA定量高灵敏度和特异性。

实验方案

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。

Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.

基因表达数据的分析需要稳定的参考或上样控制。所述参考通常是一个或多个参照基因。

查看所有结果

相关内容

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

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