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Merck
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KCQS03

KiCqStart® SYBR® Green qPCR ReadyMix预混液™

iQ™, with fluorescein for Bio-Rad systems

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UNSPSC代码:
41106300
NACRES:
NA.55

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表单

liquid

用途

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

特点

dNTPs included
hotstart

储存条件

protect from light

技术

qPCR: suitable

颜色

colorless

输入

purified DNA

相容性

for use with Bio-Rad MyiQ
for use with Bio-Rad iCycler iQ
for use with Bio-Rad iQ 5

检测方法

SYBR® Green

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

KiCqStart SYBR Green qPCR ReadyMix是2倍浓缩的即用型预混液，含有除引物和模板之外的实时定量PCR(qPCR)所需的全部组分。这种专有缓冲液、稳定剂和Taq DNA热启动聚合酶的独特组合使用快速或常规的SYBR Green qPCR循环方案，具有最高的PCR效率、灵敏度、特异性和稳定可靠的荧光信号。

高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要，因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前，处于抗体介导的失活状态。

应用

PCR扩增：
  • 基因表达
  • DNA定量
  • 染色质免疫沉淀(CHiP)
KiCqStart® SYBR® Green qPCR ReadyMix™ has been used for the qPCR analysis of 16S rRNA.
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.

特点和优势

  • 只需33分钟即可获得分析结果
  • 高效、灵敏的实时PCR结果
  • 几乎不需要多少优化，甚至无需优化

其他说明

储存条件：
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存，可稳定存放1年。为方便使用，也可在+2至+8°C下储存长达6周。解冻后，充分混匀后再使用。不建议反复冻融产品。不过，冻融20次或+20°C存放2个月后，产品没有出现任何性能损失。
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

法律信息

KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
iQ is a trademark of Bio-Rad Laboratories, Inc.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
Q66186677
Q15588
Q15324
Q15323
Q15190

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商品

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

实验方案

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Primer Optimization Using Temperature Gradient

Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.

qPCR Efficiency Determination Protocol

Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.

qPCR Gene Expression Protocol Using SYBR Green

SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.

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