ReadyScript^® cDNA 合成混合物

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.

Sigma Imprint RNA 免疫沉淀试剂盒用于从 HeLa 细胞中共纯化人 argonaute 2 (Ago2) 相关 RNA。MicroRNA通过Mysticq 试剂进行逆转录和定量。

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。

逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战，例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。

Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.

PCR是分子生物学的核心技术，功能强大。可在体外高效快速地对不同来源的特定DNA或RNA序列进行酶扩增。

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.