D8187
JumpStart™ REDTaq® DNA Polymerase
Hot-start Taq enzyme with inert dye, 10X buffer included
别名:
Hot start DNA polymerase, Hot start Taq
产品名称
JumpStart™ REDTaq® DNA Polymerase, Hot-start Taq enzyme with inert dye, 10X buffer included
form
liquid
usage
sufficient for 250 reactions
sufficient for 2500 reactions
sufficient for 50 reactions
feature
dNTPs included: no
hotstart
concentration
1 unit/μL
technique(s)
PCR: suitable
color
red
input
purified DNA
suitability
suitable for PCR
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
- insulin-enterotoxin ricin fusion gene (INS-RTB)
- endothelial cells DNA derived from reverse transcribed RNA
- leg genomic DNA from cricket flies
- mitochondrial gene by conventional PCR
Features and Benefits
- Reduces non-specific amplification
- Increased target yield and specificity
- Higher the amplification irrespective of the target concentration
- Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
- Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
- Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
- Assembled PCR reactions can be placed at room temperature for up to 2 hours
General description
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
Legal Information
Other Notes
Packaging
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
商品
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.
热启动PCR的目的在于抑制PCR反应，从而减少非特异性扩增、防止引物二聚体形成并提高产物产量。
了解聚合酶链反应（PCR）的历史，从促进它的发现的基本原理到获得诺贝尔化学奖，以及实时PCR（qPCR）和数字PCR等最近的发展。
实验方案
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
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