一般描述
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
应用
- insulin-enterotoxin ricin fusion gene (INS-RTB)
- endothelial cells DNA derived from reverse transcribed RNA
- leg genomic DNA from cricket flies
- mitochondrial gene by conventional PCR
特点和优势
- Reduces non-specific amplification
- Increased target yield and specificity
- Higher the amplification irrespective of the target concentration
- Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
- Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
- Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
- Assembled PCR reactions can be placed at room temperature for up to 2 hours
包装
单位定义
其他说明
法律信息
相关产品
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
商品
了解聚合酶链反应（PCR）的历史，从促进它的发现的基本原理到获得诺贝尔化学奖，以及实时PCR（qPCR）和数字PCR等最近的发展。
Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
热启动PCR的目的在于抑制PCR反应，从而减少非特异性扩增、防止引物二聚体形成并提高产物产量。
实验方案
介绍RedTaq的应用和优势，包括用于基因组DNA PCR的标准RedTaq、热启动RedTaq和RedTaq。
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
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