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W1754

Sigma-Aldrich

水

PCR Reagent, suitable for PCR

别名:

H2O

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10 G
¥1,590.46
100 G
¥9,752.37

¥1,590.46

国内现货，预计发货时间2025年4月07日详情

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10 G
¥1,590.46
100 G
¥9,752.37

About This Item

线性分子式:
H2O
CAS号:
7732-18-5
分子量:
18.02
Beilstein:
2050024
EC 号:
231-791-2
MDL编号:
MFCD00011332
UNSPSC代码:
12191602
PubChem化学物质编号:
24900779
NACRES:
NA.25

¥1,590.46

国内现货，预计发货时间2025年4月07日详情

产品名称

水, PCR Reagent

蒸汽密度

<1 (vs air)

蒸汽压

3 mmHg

无菌性

sterile-filtered

表单

liquid

包装

vial of 1.5 mL

技术

PCR: suitable

折射率

n20/D 1.34 (lit.)

pH值（酸碱度）

5-7

沸点

100 °C (lit.)

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D-(+)-Glucose suitable for mouse embryo cell culture, &#8805;99.5% (GC)

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D-(+)-Glucose powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, &#8805;99.5%

G7021

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D-(+)-Glucose Hybri-Max&#8482;, powder, BioReagent, suitable for hybridoma

G5146

D-(+)-Glucose

D-(+)-Glucose &#8805;99.5% (GC), BioXtra

G7528

D-(+)-Glucose

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

gas chromatography (GC): suitable

solubility

H2O: 2g + 15 mL, clear, colorless

solubility

H2O: 2g + 15 mL (Tested)

solubility

H2O: 2g + 15 mL (Tested)

solubility

H2O: 1 M

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

mp

150-152 °C (lit.)

mp

150-152 °C (lit.)

mp

150-152 °C (lit.)

mp

150-152 °C (lit.)

一般描述

对 PCR 级水进行无菌过滤。它不含核酸外切酶（dna 酶、rna 酶）和核酸内切酶（切口酶），也不含核酸污染。

应用

水已用于：

  • 作为反应混合物的组分和微流控RT-qPCR的稀释剂[1]
  • 作为反应混合物的组分，以扩增真菌（杂色云芝）DNA的产物[2]
  • 作为稀释剂和反应混合物的组分，以扩增cDNA[3]
  • 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积[3]

适用性

适用于聚合酶链式反应 (PCR)

其他说明

便于将水产品规格与 水规格表进行比较。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    SLCP8635
    SLCQ7402
    SLCQ8885
    SLCN7961
    SLCP3569

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    J Loeffler et al.
    Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
    Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
    Francine Marciano-Cabral et al.
    Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
    The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
    N Wellinghausen et al.
    Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
    Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the
    Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
    Matthew J Ellington et al.
    The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
    Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
    Bolton DJ, et al.
    Journal of Applied Microbiology, 111(2), 484-490 (2011)
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