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About This Item
线性分子式:
(CH3)3N+CH2COO−
CAS号:
分子量:
117.15
Beilstein:
3537113
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.52
一般描述
甜菜碱也称为三甲基甘氨酸或N,N,N三乙基乙酸铵，是具有三个甲基的甘氨酸类似物。这种成分高度兼容聚合酶链式反应(PCR)缓冲液混合物。甜菜碱是一种PCR增强试剂，广泛用于提高PCR产物产量和特异性——尤其是在PCR扩增高GC含量的靶标，或PCR扩增形成二级结构、导致产量不佳的靶标过程中。甜菜碱可促进DNA链分离，并管理DNA中GC和AT对之间的DNA熔化温度(Tm)差异。它通过平衡GC-和AT-碱基对的贡献来稳定ds DNA。甜菜碱广泛用于优化多重和“长链且精确”的聚合酶链式反应（LA-PCR）。据报道，向PCR混合物中添加1.0-1.7M甜菜碱水溶液，可减少碱基对组成对DNA链解链的依赖性。
应用
甜菜碱溶液已被用作以下反应混合物的成分:
- 环介导等温扩增 (LAMP) 反应
- 用于基因组 DNA 扩增的聚合酶链反应
- 用于端粒长度分析的定量聚合酶链反应 (qPCR)
- 基因组 DNA 中 CGG 重复序列的 PCR 扩增
- 用于检测严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2)的逆转录环介导的等温扩增(RT-LAMP)
- DNA 定向 RNA 聚合酶 II 最大亚基(RPB2) 的 PCR 扩增
- 文库制备过程使用 Smartseq2 进行逆转录
包装
每管1.5mL
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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商品
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
实验方案
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.
相关内容
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
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