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Merck
CN

B0300

甜菜碱 溶液

5 M, PCR Reagent

别名:

TMG, trimethylglycine

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关于此项目

线性分子式:
(CH3)3N+CH2COO−
化学文摘社编号:
107-43-7
分子量:
117.15
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24891511
MDL number:
MFCD00012123
Beilstein/REAXYS Number:
3537113

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产品名称

甜菜碱 溶液, 5 M, PCR Reagent

InChI

1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3

SMILES string

C[N+](C)(C)CC([O-])=O

InChI key

KWIUHFFTVRNATP-UHFFFAOYSA-N

form

liquid

packaging

vial of 1.5 mL

concentration

5 M

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

Quality Level

300

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Application

甜菜碱溶液已被用作以下反应混合物的成分:
  • 环介导等温扩增 (LAMP) 反应
  • 用于基因组 DNA 扩增的聚合酶链反应
  • 用于端粒长度分析的定量聚合酶链反应 (qPCR)
  • 基因组 DNA 中 CGG 重复序列的 PCR 扩增
  • 用于检测严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2)的逆转录环介导的等温扩增(RT-LAMP)
  • DNA 定向 RNA 聚合酶 II 最大亚基(RPB2) 的 PCR 扩增
  • 文库制备过程使用 Smartseq2 进行逆转录

General description

甜菜碱也称为三甲基甘氨酸或N,N,N三乙基乙酸铵，是具有三个甲基的甘氨酸类似物。这种成分高度兼容聚合酶链式反应(PCR)缓冲液混合物。甜菜碱是一种PCR增强试剂，广泛用于提高PCR产物产量和特异性——尤其是在PCR扩增高GC含量的靶标，或PCR扩增形成二级结构、导致产量不佳的靶标过程中。甜菜碱可促进DNA链分离，并管理DNA中GC和AT对之间的DNA熔化温度(Tm)差异。它通过平衡GC-和AT-碱基对的贡献来稳定ds DNA。甜菜碱广泛用于优化多重和“长链且精确”的聚合酶链式反应（LA-PCR）。据报道，向PCR混合物中添加1.0-1.7M甜菜碱水溶液，可减少碱基对组成对DNA链解链的依赖性。

Packaging

每管1.5mL

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000518569
0000518569
0000514975
0000514975
0000514017

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P N Hengen
Trends in biochemical sciences, 22(6), 225-226 (1997-06-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and
Thanyaornwanya Charoenwijitkul et al.
Clinical biochemistry, 71, 31-37 (2019-06-15)
The 3.7 kb deletion (-α3.7) is the most common form of α+-thalassemia found in multiple populations which can be classified into three subtypes. In order not to mis-identify it, the molecular information within each population is required. We have addressed this
Betaine chemistry, roles, and potential use in liver disease
Day CR and Kempson SA
Biochimica et biophysica acta. General subjects, 1860(6), 1098-1106 (2016)
Thirty-minute screening of antibiotic resistance genes in bacterial isolates with minimal sample preparation in static self-dispensing 64 and 384 assay cards
Kostic T, et al.
Applied Microbiology and Biotechnology, 99(18), 7711-7722 (2015)
DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesis
Jensen, Michael A and Fukushima, Marilyn and Davis, Ronald W
PLoS ONE, 5(6), e11024-e11024 (2010)
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Product Information Sheet - B0300

Instructions

Optimizing Your Genotyping Workflow
User Guide

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